環境DNA論文について

Ficetolaが2008年に環境DNAでウシガエルを検出した事をきっかけに、環境DNAの検出技術は特定の種のみならず、複数種の同時検出が可能となりました。また、近年ではさらに生物の状態を反映するRNAの検出ができるようになってきています。

全世界で活発に研究されている環境DNAについて少しでも理解しやすくなるようにこのページを作りました。ぜひ仕事や研究に有効活用していただければと思います。時々更新します。

2008年以降の環境DNA論文数の推移

大型脊椎動物を対象とした環境DNAメタバーコーディングが使用され始めた2014年ごろから論文公開本数が上昇傾向にあります。メタバーコーディングを使って生物相を検出する研究が多くなったからでしょうか。

2020年の環境DNA論文数の推移

2020年は月平均で16本程度の環境DNA論文が全世界を通して公開されているようです。

最終更新: 2020/10/22

環境DNA論文のサマリーシート(2020年)

2020年限定ですが簡単な訳をつけて環境DNA論文を日本語で検索できるようにしました。

最終更新: 2020/10/22

No.	Title	和訳(DeepL+意訳)	Author
1	Using environmental DNA analysis to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis	環境DNA分析を使用して絶滅危惧種である両側回遊性のリュウキュウアユの出現と個体数の評価	Akamatsu et al.
2	Uncovering the complete biodiversity structure in spatial networks – the example of riverine systems	空間ネットワークの完全な生物多様性構造の解明–河川システムの例	Altermatt et al.
3	Steps towards a more efficient use of chironomids as bioindicators for freshwater bioassessment: Exploiting eDNA and other genetic tools	淡水生物評価のバイオ指標としてのユスリカのより効率的な使用に向けた歩み：eDNAおよびその他の遺伝的ツールの活用	Czechowski et al.
4	Environmental DNA reveals seasonal shifts and potential interactions in a marine community	環境DNAは季節的な変化と海洋コミュニティの潜在的な相互作用を明らかにする	Djurhuus et al.
5	Detection of freshwater mussels (Unionidae) using environmental DNA in riverine systems	河川システムにおける環境DNAを使用したカラスガイ(イシガイ科)の検出	Gasparini et al.
6	Finding Crush: Environmental DNA Analysis as a Tool for Tracking the Green Sea Turtle Chelonia mydas in a Marine Estuary	Finding Crush：海洋河口のアオウミガメChelonia mydasを追跡するためのツールとしての環境DNA分析	Harper et al.
7	Use of environmental DNA (eDNA) in streams to detect feral swine (sus scrofa)	野生ブタ(イノシシ)を検出するための河川での環境DNA(eDNA)の使用	Hauger et al.
8	From eDNA to citizen science: emerging tools for the early detection of invasive species	環境DNAから市民科学まで：侵入種の早期発見のための新しいツール	Larson et al.
9	A comparison of eDNA to camera trapping for assessment of terrestrial mammal diversity	陸棲哺乳類の多様性評価のためのカメラトラップと環境DNAの比較	Leempoel et al.
10	Multiple lines of genetic inquiry reveal effects of local and landscape factors on an amphibian metapopulation	複数の遺伝的調査は両生類のメタ個体群に対する局所的および景観的要因の影響を明らかにする	Parsley et al.
11	The future of fish-based ecological assessment of European rivers: from traditional EU Water Framework Directive compliant methods to eDNA metabarcoding-based approaches	欧州の河川の魚に基づく生態学的評価の未来：従来のEU水枠組み指令に準拠した方法から環境DNAメタバーコーディングに基づくアプローチまで	Pont et al.
12	Analytical validation and field testing of a specific qPCR assay for environmental DNA detection of invasive European green crab (Carcinus maenas)	侵略的ヨーロッパミドリガニ(Carcinus maenas)の環境DNA検出のための特異的qPCRの分析検証と現場試験	Roux et al.
13	Assessing the potential of environmental DNA metabarcoding for monitoring Neotropical mammals: a case study in the Amazon and Atlantic Forest, Brazil		Sales et al.
14	Increased eDNA detection sensitivity using a novel highvolumewater sampling method	新規的な大容量水サンプリング法を用いた環境DNAの検出感度の増加	Schabacker et al.
15	A round-robin evaluation of the repeatability andreproducibility of environmental DNA assays for dreissenid mussels	ムラサキイガイの環境DNA評価の反復性と再現性のラウンドロビン評価	Sepulveda et al.
16	A mesocosm comparison of laboratory-based and on-site eDNA solutions for detection and quantification of striped bass (Morone saxatilis) in marine ecosystems	海洋生態系におけるストライプバス(Morone saxatilis)の検出と定量化のための、実験室ベースとオンサイトでの環境DNA法によるメソコスム比較	Skinner et al.
17	Experimental assessment of optimal lotic eDNA sampling and assay multiplexing for a critically endangered fish	絶滅の危機に瀕する魚類のための局所的な環境DNAサンプリングと複数の検証法	Wood et al.
18	Development of a TaqMan qPCR protocol for detecting Acipenser ruthenus in the Volga headwaters from eDNA samples	ボルガ源流域におけるAcipenser ruthenusを環境DNAサンプルから検出するためのTaqMan qPCRプロトコルの開発	Schenekar et al.
19	Environmental DNA‑based xenomonitoring for determining Schistosoma presence in tropical freshwaters	熱帯淡水域における 住血吸虫属の存在を判定するための環境DNAベースの異種生物モニタリング	Alzaylaee_et al.
20	Spatial and temporal patterns of environmental DNA detection to inform sampling protocols in lentic and lotic systems	流域および止水域のサンプリングプロトコルに情報を与えるための環境DNA検出の空間的・時間的パターン	Bedwell et al.
21	Groundtruthing of pelagic forage fish detected by hydroacoustics in a whale feeding area using environmental DNA	環境DNAを用いたクジラ餌場のハイドロアコースティクスで検出された遠洋性採餌魚のグランドトゥルーチング	Berger et al.
22	Maximizing fish detection with eDNA metabarcoding	環境DNAメタバーコーディングによる魚群探知の最大化	Bessey et al.
23	Exploring Environmental DNA (eDNA) to Assess Biodiversity of Hard Substratum Faunal Communities on the Lucky Strike Vent Field (Mid-Atlantic Ridge) and Investigate Recolonization Dynamics After an Induced Disturbance	ラッキーストライクベントフィールド（中大西洋海嶺）における硬質基質動物群集の生物多様性を評価するための環境DNA（eDNA）の探索と撹乱後の再コロニー化ダイナミクスの調査水生環境DNAのサンプル前処理とPCR前処理の実践ガイド	Cowart et al.
24	A practival guide to sample presrvation and pre-PCR processing of aquatic environmental DNA	水生環境DNAのサンプル前処理とPCR前処理の実践ガイド	Kumer et al.
25	Are migratory waterfowl vectors of seagrass pathogens?	渡り鳥の水鳥は海草の病原体の媒介者なのか？	Menning et al.
26	Space invaders: Searching for invasive Smallmouth Bass (Micropterus dolomieu) in a renowned Atlantic Salmon (Salmo salar) river	宇宙の侵略者。アトランティックサーモン（Salmo salar）の有名な河川での侵略的スモールマウスバス（Micropterus dolomieu）の探索	O’Sullivan et al.
27	Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA	堆積物の環境DNAは、水中の環境DNAと比較して、時間スケールや魚種の組成について異なる情報を提供する	Sakata et al.
28	Complementary molecular methods reveal comprehensive phylogenetic diversity integrating inconspicuous lineages of early-diverged wood-decaying mushrooms	補完的分子法を用いて、早発木質腐朽キノコの隠れた系統を統合した系統多様性の解明	Shirouzu et al.
29	Identifying conservation priorities in a defaunated tropical biodiversity hotspot	衰退した熱帯の生物多様性ホットスポットにおける保全の優先順位の特定	Tilker et al.
30	Novel universal primers for metabarcoding environmental DNA surveys of marine mammals and other marine vertebrates	海洋哺乳類およびその他の海洋脊椎動物の環境DNAメタバーコーディング調査のための新規ユニバーサルプライマーの開発	Valsecchi et al.
32	Analysis of a coastal North Sea fish community: Comparison of aquatic environmental DNA concentrations to fish catches	北海沿岸の魚類群集の解析 : 水生環境DNA濃度と漁獲量の比較	van Bleijswijk et al.
33	Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA (eDNA) metabarcoding	環境DNA(eDNA)メタバーコーディングを用いた陸域生物多様性モニタリングのための複数の基盤的なテスト	Van der Heyde
34	Holistic pelagic biodiversity monitoring of the Black Sea via eDNA metabarcoding approach: From bacteria to marine mammals	環境DNAメタバーコーディングアプローチによる黒海の遠洋生物多様性モニタリング : バクテリアから海洋哺乳類まで	Zhang et al.
35	Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: Development and application of a species-specific molecular assay	アイルランドの湖沼における北極イワナ(Salvelinus alpinus)の環境DNA検出 : 種特異的分子分析法の開発と応用	Mirinmin et al.
36	Moving eDNA surveys onto land: Strategies for active eDNA aggregation to detect invasive forest insects	環境DNA調査を陸上 : 侵略的な森林昆虫を検出するための能動的な環境DNAアグリゲーションの戦略	Valentin et al.
37	DNA and eDNA-based tracking of the North African sharptooth catfish Clarias gariepinus	北アフリカのsharptooth catfish(Clarias gariepinus)のDNAとeDNAに基づく追跡	Elberri et al.
38	Advances and prospects of environmental DNA in neotropical rainforests	新熱帯雨林における環境DNAの進歩と展望	Zinger et al.
39	Environmental DNA sampling informs fish eradication efforts: case studies and lessons learned	環境DNAサンプリングが魚類の根絶に役立つ：事例研究と教訓	Carim et al.
40	Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA	環境DNAを用いたquaggaおよびzebra mussels(Dreissena rostriformis bugensisおよびD. polymorpha)の検出のための単純かつ高感度で種特異的な分析法	Blackman et al.
41	Standards for Methods Utilizing Environmental DNA for Detection of Fish Species	魚類の種の検出に環境DNAを利用した方法の基準	Shu et al.
42	Environmental DNA (eDNA) detection of marine aquatic invasive species (AIS) in Eastern Canada using a targeted species-specific qPCR approach	標的化された種特異的qPCR法を用いたカナダ東部の海洋水生侵略種(AIS)の環境DNA(eDNA)検出	LeBlanc et al.
43	Reliable eDNA detection and quantification of the European weather loach (Misgurnus fossilis)	Msgurnus fossilisの信頼性の高い環境DNA検出と定量化	Brys et al.
44	Genetic tools in the management of invasive mammals: recent trends and future perspectives	侵略的哺乳類の管理における遺伝子ツール：最近の傾向と将来の展望	Browett et al.
45	A muddy time capsule: using sediment environmental DNA for the long-term monitoring of coastal vegetated ecosystems	泥だらけのタイムカプセル：沿岸植生生態系の長期モニタリングのための堆積物環境DNAの利用	Forster et al.
46	Revising the range of Rocky Mountain tailed frog, Ascaphus montanus, in British Columbia, Canada, using environmental DNA methods	環境DNA法を用いたカナダ・ブリティッシュコロンビア州のAscaphus montanusの生息域の見直し	Hobbs et al.
47	Validating metabarcoding-based biodiversity assessments with multi-species occupancy models: A case study using coastal marine eDNA	多種占有モデルを用いたメタバーコーディングに基づく生物多様性評価の検証 沿岸海域の海洋環境DNAを用いたケーススタディ	McClenaghan et al.
48	Validating environmental DNA metabarcoding for marine fishes in diverse ecosystems using a public aquarium	水族館を利用した多様な生態系における海洋魚類の環境DNAメタバーコーディングの検証	Morey et al.
49	Detection of lamprey in Southernmost South America by environmental DNA (eDNA) and molecular evidence for a new species	環境DNAによる南米最南端のヤツメの検出と新種の分子的証拠	Nardi et al.
50	Are bacteria potential sources of fish environmental DNA?	バクテリアは魚の環境DNAの潜在的な供給源になるのか？	Nukazawa et al.
51	Fishing for mammals: Landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems	哺乳類を釣る: 河川系の環境DNAを用いた陸生・半水生生物群集の景観レベルでのモニタリング	Sales et al.
52	Parallel, targeted analysis of environmental samples via highthroughput quantitative PCR	ハイスループット定量PCRによる環境試料のパラレル・ターゲット解析	Wilcox et al.
53	Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples	海水試料中の外来種Ectopleura crocea (Hydrozoa: Anthoathecata)を検出するための環境DNAと特異的プライマーの開発	Kim et al.
54	Detecting Native Freshwater Fishes Using Novel Non-invasive Methods	新しい非侵襲的手法を用いた在来淡水魚の検出]	Castaneda et al.
55	The effect of temperature on environmental DNA degradation of Japanese eel	ニホンウナギの環境DNAの分解に及ぼす温度の影響	Kasai et al.
56	Enhancing tropical conservation and ecology research with aquatic environmental DNA methods: an introduction for non-environmental DNA specialists	水生環境DNA法による熱帯保全・生態学研究の強化：非環境DNA専門家のための入門書	Huerlimann et al.
57	Organic matter reduces the amount of detectable environmental DNA in freshwater	有機物は淡水中の検出可能な環境DNA量を減少させる	Bochove et al.
58	Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs	ダム貯水池における魚類環境DNAメタバーコーデングに及ぼすサンプリング季節性と場所の影響	Hayami et al.
59	Chapter Ten – Informing marine spatial planning decisions with environmental DNA	第10章 環境DNAを用いた海洋空間計画の意思決定の情報化	Bani et al.
60	Validation of an environmental DNA protocol to detect a stream‐breeding amphibian, the Streamside Salamander (Ambystoma barbouri)	流域で繁殖する両生類であるStreamside Salamander（Ambystoma barbouri）を検出するための環境DNAプロトコルの検証	Witzel et al.
61	Detecting invertebrate ecosystem service providers in orchards: traditional methods versus barcoding of environmental DNA in soil	果樹園における無脊椎動物の生態系サービス提供者の検出：従来の方法と土壌中の環境DNAのバーコーディングの比較	Todd et al.
62	Qualitative and quantitative detection using eDNA technology: A case study of Fenneropenaeus chinensis in the Bohai Sea	eDNA技術を用いた定性・定量的検出 : Bohai海域におけるコウライエビのの事例研究	Li et al.
63	Dead or alive: sediment DNA archives as tools for tracking aquatic evolution and adaptation	生か死か：水生生物の進化と適応を追跡するためのツールとしての堆積物DNAアーカイブ	Ellegaard
64	Analysis of sea lamprey environmental DNA during lampricide treatment in a tributary of Lake Ontario	オンタリオ湖の支流におけるlampricede処理中のウミヤツメ環境DNA分析	Kaitlyn et al.
65	Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA	環境DNAを用いた米国西テキサス州における両生類病原体のChytrid Fungus（Batrachochytrium dendrobatidis）とRanavirusの検出	Barnes et al.
66	Supervised machine learning is superior to indicator value inference in monitoring the environmental impacts of salmon aquaculture using eDNA metabarcodes	教師付き機械学習は環境DNAメタバーコーディングを用いたサケの繁殖環境モニタリング時の指標値推論において優れている	Frühe et al.
67	Identifying error and accurately interpreting eDNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes	誤差の特定とeDNAメタバーコーディング結果の正確な解釈 : 乾燥地帯の水飲み場における脊椎動物検出のためのケーススタディ	Furlan et al.
68	Environmental DNA analysis shows high potential as a tool for estimating intraspecific genetic diversity in a wild fish population	環境DNA分析は野外魚類個体群の種内遺伝的多様性を推定するツールとして高い可能性を示す	Tsuji et al.
69	Estimations of Riverine Distribution, Abundance, and Biomass of Anguillid Eels in Japan and Taiwan Using Environmental DNA Analysis	環境DNA解析を用いた日本と台湾におけるAnguillid Eelsの河川分布・量・バイオマスの推定
70	Environmental DNA metabarcoding for freshwater bivalves biodiversity assessment: methods and results for the Western Palearctic (European sub-region)	淡水二枚貝の生物多様性評価のための環境DNAメタバーコーディング : 西南極海(ヨーロッパ亜地域) の方法と結果	Prié et al.
71	Monitoring the silver carp invasion in Africa: a case study using environmental DNA (eDNA) in dangerous watersheds	アフリカにおけるSilver carpの侵入監視：危険な流域における環境DNA(eDNA)を用いた事例研究	Crookes et al.
72	Using environmental DNA to detect estuarine crocodiles, a cryptic-ambush predator of humans	環境DNAを用いた人を捕食するワニを河口域から検出する
73	Using environmental DNA to monitor the spatial distribution of the California Tiger Salamander Ambystoma californiense	環境DNAを用いたCalifornia Tiger Salamander(Ambystoma californiense)の空間分布のモニタリング	Kieran et al.
74	A drop in the ocean: Monitoring fish communities in spawning areas using environmental DNA	海中の中の一滴 : 環境DNAを用いた産卵地域の魚類群集モニタリング	Ratcliffe et al.
75	Are Environmental DNA Methods Ready for Aquatic Invasive Species Management?	環境DNA法は水生外来種管理のための準備ができているか？	Sepulveda et al.
76	eDNA and metabarcoding for rewilding projects monitoring, a dietary approach	再野生化プロジェクトのモニタリングのための環境DNAとメタバーコーディング、食事的アプローチ
77	Improved Environmental DNA Reference Library Detects Overlooked Marine Fishes in New Jersey, United States	改良された環境DNAリファレンスライブラリーは米国ニュージャージー州の見落とされた海洋魚類を検出する	Stoeckle et al.
78	Caged fish experiment and hydrodynamic bidimensional modeling highlight the importance to consider 2D dispersion in fluvial environmental DNA studies	ケージ内の魚類を使った実験と流体力学的2次元モデリングは河川環境DNAにおける2次元分散を考慮することの重要性を強調する	Laporte et al.
79	Molecular and morphological signatures for extreme environmental adaptability of the invasive mussel Brachidontes pharaonis	侵略的な貝(Brachidontes pharaonis)の極端な環境適応性に関する分子学的・形態学的特徴	Mohammed-Geba et al.
80	Monitoring freshwater fish communities in large rivers using environmental DNA (eDNA) metabarcoding and a long‐term electrofishing survey	環境DNA(eDNA)メタバーコーディングと長期の電気漁法を用いた大河川における魚類群集のモニタリング	Goutte et al.
81	Environmental DNA can act as a biodiversity barometer of anthropogenic pressures in coastal ecosystems	環境DNAは沿岸生態系における人為的な圧力の生物多様性のバロメーターとして機能する	DiBattista et al.
82	Macroinvertebrate community diversity and habitat quality relationships along a large river from targeted eDNA metabarcode assays	標準的な環境DNAメタバーコーディング評価に基づく大河川の大型脊椎動物群集の多様性と生息地の質の関係	Marshall et al.
83	A comparison of European eel Anguilla anguilla eDNA concentrations to fyke net catches in five Irish lakes	アイルランドの5つの湖におけるネット漁法とヨーロッパウナギ(Anguilla anguilla)の環境DNA濃度の比較	Weldon et al.
84	Quantitative evaluation of intraspecific genetic diversity in a natural fish population using environmental DNA analysis	環境DNA分析による天然魚類個体群の種内遺伝的多様性の定量的な評価	Tsuji et al.
85	Optimizing an eDNA protocol for estuarine environments: Balancing sensitivity, cost and time	河口域環境での環境DNAプロトコルの最適化: 感度、コスト、時間のバランス	Sanches et al.
86	eDNA‐based monitoring: Advancement in management and conservation of critically endangered killifish species	環境DNAによるモニタリング: 絶滅危機のメダカの管理と保全の進展	Mauvisseau et al.
87	Identifying Under‐Ice Overwintering Locations of Juvenile Chinook Salmon by Using Environmental DNA	環境DNAを用いたChinookサーモンの幼魚の氷下の越冬場所の特定	Khalsa et al.
88	60 specific eDNA qPCR assays to detect invasive, threatened, and exploited freshwater vertebrates and invertebrates in Eastern Canada	カナダ東部で侵略され、絶滅の危機に晒され、悪用されている淡水の脊椎動物・無脊椎動物を検出するための60の特異的な環境DNAqPCR評価法	Hernandez et al.
89	Environmental DNA reveals cryptic diversity within the subterranean amphipod genus Pseudocrangonyx Akatsuka & Komai, 1922 (Amphipoda: Crangonyctoidea: Pseudocrangonyctidae) from central Japan	環境DNAから明らかになった地中の端脚類の多様性(Pseudocrangonyx Akatsuka & Komai, 1922)の多様性	Yonezawa et al.
90	Comparing diversity levels in environmental samples: DNA sequence capture and metabarcoding approaches using 18S and COI genes	環境試料における生物多様性レベルの比較: 18sとCOI領域を用いたDNA配列の捕捉とメタバーコーディングによるアプローチ	Langen et al.
91	Review and application of environmental DNA (eDNA) investigation of terrestrial species in urban ecosystem	都市生態系における陸棲種の環境DNA(eDNA)調査の検討と応用	Kim et al.
92	Environmental DNA: A promising factor for tuberculosis risk assessment in multi-host settings	環境DNA: 複数の宿主環境における結核リスク評価のための有望な要因	Martínez-Guijosa et al.
93	Application of eDNA as a tool for assessing fish population abundance	魚類個体群の豊富さを評価するためのツールとしての環境DNAの応用	Spear et al.
94	Two Ocean Pass: An Alternative Hypothesis for the Invasion of Yellowstone Lake by Lake Trout, and Implications for Future Invasions	Two Ocean Pass: イエローストーン湖におけるレイクトラウトの侵入に関する代替仮説と将来の侵入への示唆	Koel et al.
95	Detection of herbivory: eDNA detection from feeding marks on leaves	草食の検出：葉の摂食痕からのeDNA検出	Aoi et al.
96	No evidence that crayfish carcasses produce detectable environmental DNA (eDNA) in a stream enclosure experiment	ザリガニの死骸が検出可能な環境DNA(eDNA)を生成するという証拠はない	Curtis et al.
97	Aquatic insect community structure revealed by eDNA metabarcoding derives indices for environmental assessment	環境DNAメタバーコーディングにより明らかになった水生昆虫群集構造は環境評価の指標となる	Uchida et al.
98	Leveraging eDNA to expand the study of hybrid zones	ハイブリッドゾーンの研究を拡大するための環境DNAの活用	Stewart et al.
99	Modeling the ecological status response of rivers to multiple stressors using machine learning: A comparison of environmental DNA metabarcoding and morphological data	機械学習を用いた複数のストレス要因に対する河川の生態状態応答のモデル化: 環境DNAメタバーコーディングと形態学的データの比較	Fan et al.
100	Pathway to Increase Standards and Competency of eDNA Surveys (PISCeS)—Advancing collaboration and standardization efforts in the field of eDNA	環境DNA調査の標準化・能力向上のための道筋（PISCeS）-環境DNA分野における標準化・共同研究の推進	Loeza-Quintana et al.
101	Simultaneous detection of native and invasive crayfish and Aphanomyces astaci from environmental DNA samples in a wide range of habitats in Central Europe	中央ヨーロッパの広範な生息地における環境DNAサンプルからの在来ザリガニと侵入ザリガニおよびAphanomyces astaciの同時検出	Rusch et al.
102	Development and evaluation of fish eDNA metabarcoding assays facilitate the detection of cryptic seahorse taxa (family: Syngnathidae)	魚類の環境DNAメタバーコーデングの評価法はタツノオトシゴ属の検出を容易にする	Nester et al.
103	Estimating fish population abundance by integrating quantitative data on environmental DNA and hydrodynamic modeling	環境DNAの定量データと流体モデルの統合による魚類個体数の推定	Fukaya et al.
104	Can environmental DNA be used to detect first arrivals of the cane toad, Rhinella marina , into novel locations?	環境DNAを用いてオオヒキガエル(Rhinella marina)の新生息地への到達を検出可能か？	Villacorta-Rath et al.
105	Droplet digital PCR applied to environmental DNA, a promising method to estimate fish population abundance from humic‐rich aquatic ecosystems	フミン酸リッチな水棲生態系における環境DNAとデジタルPCRを用いた魚類の個体数推定法の開発	Capo et al.
106	Executing multi-taxa eDNA ecological assessment via traditional metrics and interactive networks	従来のメトリクスと対話型ネットワークを用いた複数分類群の環境DNAによる生態学的評価の実行	Seymour et al.
107	Use of Environmental DNA to Determine Fantail Darter (Etheostoma flabellare) Density in a Laboratory Setting: Effects of Biomass and Filtration Method	実験室環境におけるFantail Darter(Etheostoma flabellare)の密度を決定するための環境DNAの利用: バイオマスとろ過方法の効果	Guivas et al.
108	Exploration of Environmental DNA (eDNA) to Detect Kirtland’s Snake (Clonophis kirtlandii)	Kirhland’s Snake(Clonophis kirtlandii)を検出するための環境DNAの探索	Ratsch et al.
109	Environmental DNA from plastic and textile marine litter detects exotic and nuisance species nearby ports	港付近に生息する外来種と有害種をプラスチックや繊維質の海洋ごみ由来の環境DNAから検出する	Ibabe et al.
110	Capture of Environmental DNA (eDNA) from Water Samples by Flocculation.	凝集による水試料からの環境DNAの捕集	Schill et al.
111	Characterizing the spatial and temporal occurrence patterns of the endangered botiid loach Parabotia curtus by environmental DNA analysis using a newly developed species-specific primer set	新規開発の種特異的プライマーセットを用いた環境DNA解析による絶滅の危機に瀕するドジョウ(Parabotia curtus)の空間的・時間的発生パターン解析	Sugiura et al.
112	Accumulation curves of environmental DNA sequences predict coastal fish diversity in the coral triangle	環境DNA配列の累積曲線はサンゴデルタ地帯の沿岸魚類相の多様性を予測する	Juhel et al.
113	The relationship between eDNA particle concentration and organism abundance in nature is strengthened by allometric scaling	自然環境中の環境DNAの粒子密度とサイズと生物の豊富さの関係は非比例的スケーリングによって補強される	Yates et al.
114	Use of environmental DNA to detect the invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes	環境DNAを用いた湖沼における侵略的水生植物 Myriophyllum spicatumとEgeria densaの検出	Kuehne et al.
115	Comparing environmental metabarcoding and trawling survey of demersal fish communities in the Gulf of St. Lawrence, Canada	カナダ・セントローレンス湾における環境メタバーコーディングと底生魚群集のトロール調査の比較	Afzali et al.
116	Environmental DNA allows upscaling spatial patterns of biodiversity in freshwater ecosystems	淡水生態系における生物多様性の空間パターンの拡大を可能にする環境DNA	Carraro et al.
117	Environmental DNA detection tracks established seasonal occurrence of blacktip sharks (Carcharhinus limbatus) in a semi-enclosed subtropical bay	半閉鎖的亜熱帯性の湾におけるBlacktip sharks(Carcharhinus limbatus)の季節的な発生を追跡する環境DNA検出法の開発	Postaire et al.
118	Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals	集められたサンプルとeDNAベースの検出は、水生動物の「クリーンな取引」を促進する	Brunner
119	Design and Validation of Passive Environmental DNA Samplers (PEDS) using Granular Activated Carbon (GAC) and Montmorillonite Clay (MC)	粒状活性炭(GAC)とモンモリロナイト粘土(MC)を用いた受動的環境DNAサンプラー(PEDS)の設計と検証	Kirtane et al.
120	Assessment of fish biodiversity in four Korean rivers using environmental DNA metabarcoding	環境DNAメタバーコーディングを用いた韓国4河川における魚類の生物多様性の評価	Alam et al.
121	An Optional Low-Cost Method of Extracting Environmental DNA of Macro-Organisms from Filter Membranes in Large Scale eDNA Surveys	大規模な環境DNA調査におけるメンブレンフィルターからの大型生物に由来する環境DNAの低コストな抽出法の検討	Chen et al.
122	Field storage of water samples affects measured environmental DNA concentration and detection	野外での水サンプルの保管は環境DNAの濃度と検出に影響を及ぼす	Curtis et al.
123	Piloting an integrated approach for estimation of environmental risk of Schistosoma haematobium infections in pre-school-aged children and their mothers at Barombi Kotto, Cameroon	Piloting an integrated approach for estimation of environmental risk of Schistosoma haematobium infections in pre-school-aged children and their mothers at Barombi Kotto, Cameroon	Eyre et al.
124	Metabarcoding unsorted kick‐samples facilitates macroinvertebrate‐based biomonitoring with increased taxonomic resolution, while outperforming environmental DNA	キックサンプルのメタバーコーディングは環境DNAより優れており、分類学的分解能の向上と大型無脊椎動物のモニタリングを可能とする	Pereira‐da‐Conceicoa et al.
125	Development of an environmental DNA metabarcoding assay for aquatic vascular plant communities	水棲の維管束植物群集の環境DNAメタバーコーディング法の開発	Coghlan et al.
126	Understanding leptospirosis eco-epidemiology by environmental DNA metabarcoding of irrigation water from two agro-ecological regions of Sri Lanka	スリランカの2つの農業生態学的地域の灌漑用水の環境DNAメタバーコーディングによるレプトスピラ症の生態疫学的な理解	Gamage et al.
127	New barcoding primers for the efficient monitoring of diplonemid diversity	ミドリムシ門の多様性を効率的にモニタリングするための新たなバーコーディングプライマー	Yabuki et al.
128	Detection of the parasitic nematode, Pseudocapillaria tomentosa, in zebrafish tissues and environmental DNA in research aquaria	ゼブラフィッシュ組織中の寄生線虫(Pseudocapillaria tomentosa)の検出と研究用水槽の環境DNAの検出	Norris et al.
129	Methods to maximize environmental DNA (eDNA) for detection the presence of Alligator Gar (Atractosteus spatula)	アリゲーターガー(Atractosteus spatula)の生息を検出するための環境DNAの能力を最大化する方法	Nur et al.
130	Development and application of eDNA-based tools for the conservation of white-clawed crayfish	white-clawed crayfishの保護のための環境DNAに基づくツールの開発と応用	Troth et al.
131	Calibrating Environmental DNA Metabarcoding to Conventional Surveys for Measuring Fish Species Richness	魚類の種多様性を測るための環境DNAメタバーコーディングを従来調査に適用する	McElroy et al.
132	Blind assessment of vertebrate taxonomic diversity across spatial scales by clustering environmental DNA metabarcoding sequences	環境DNAメタバーコーディング配列のクラスタリングによる脊椎動物分類学的多様性の空間スケールでのブラインド評価	Marques et al.
133	Marine water environmental DNA metabarcoding provides a comprehensive fish diversity assessment and reveals spatial patterns in a large oceanic area	海洋水に由来する環境DNAのメタバーコーディングは、魚類の多様性を包括的に評価し、大洋域の空間パターンを明らかにする	Fraija-Fernández et al.
134	Accounting for false positive detections in occupancy studies based on environmental DNA: A case study of a threatened freshwater fish (Galaxiella pusilla)	環境DNAに基づく占有調査における誤検出の説明: 絶滅の危機に瀕する淡水魚(Galaxiella pusilla)の事例研究	Tingley et al.
135	Pitfalls during in silico prediction of primer specificity for eDNA surveillance	環境DNAの調査のためのプライマー特異性のIn silicoにおける予測の落とし穴	So et all.
136	Remote, autonomous real-time monitoring of environmental DNA from commercial fish	一般的な魚類の環境DNAの遠隔・自律式リアルタイムモニタリング	Hansen et al.
137	An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols	環境DNA研究のための図解マニュアル: 採水ガイドラインと実験プロトコル	Minamoto et al.
138	Assessment of stream macroinvertebrate communities with eDNA is not congruent with tissue‐based metabarcoding	環境DNAを用いた河川の大型脊椎動物群集の評価は組織ベースのメタバーコーディングとは一致しない	Gleason et al.
139	Lost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNA	失われたものと発見されたもの。生物多様性ホットスポットのカエルを環境DNAで再発見	Lopes et al.
140	Honey as a Source of Environmental DNA for the Detection and Monitoring of Honey Bee Pathogens and Parasites	ミツバチの病原体や寄生虫の検出とモニタリングのための環境DNAの供給源としての蜂蜜	Ribani et al.
141	Species detection from aquatic eDNA: Assessing the importance of capture methods	水環境に由来する環境DNAから種の検出: 捕獲法の重要性を評価する	Peixoto et al.
142	BarcodingGO: A problem‐based approach to teach concepts related to environmental‐DNA and bioinformatics	BarcodingGO: 環境DNAおよびバイオインフォマティクスに関連する概念を教えるための問題提起型のアプローチ	Nunes et al.
143	Projection range of eDNA analysis in marshes: a suggestion from the Siberian salamander (Salamandrella keyserlingii) inhabiting the Kushiro marsh, Japan	湿地帯における環境DNA分析の影響範囲: 釧路湿原に生息するシベリアサンショウウオ(Salamandrella keyserlingii)による示唆	Takeshita et al.
144	Application of environmental DNA methods for the detection and abundance estimation of invasive aquatic plant Egeria densa in lotic habitats	侵襲性水生植物(Egeria densa)の検出と生息数推定のための環境DNA法の適用	Miyazono et al.
145	Status of the major aquaculture carps of China in the Laurentian Great Lakes Basin		Chapman et al.
146	Designing environmental DNA surveys in complex aquatic systems: Backpack sampling for rare amphibians in Sierra Nevada meadows	複雑な水生システムにおける環境DNA調査の設計: シエラネバダの牧草地における希少両生類のバックパックサンプリング	Pope et al.
147	Environmental DNA metabarcoding for biodiversity monitoring of a highly diverse tropical fish community in a coral reef lagoon: Estimation of species richness and detection of habitat segregation	サンゴ礁のラグーンにおける多様性の高い熱帯魚群集の生物多様性モニタリングのための環境DNAメタバーコーディング: 種多様性の推定と生息環境の分離の検出	Oka et al.
148	Fine-scale environmental heterogeneity shapes fluvial fish communities as revealed by eDNA metabarcoding	環境DNAメタバーコーディングによる河川魚類群集の微細な環境不均一性の解明	Berger et al.
149	Environmental DNA metabarcoding provides enhanced detection of the European eel Anguilla anguilla and fish community structure in pumped river catchments	環境DNAメタバーコーディングは揚水河川集水域におけるヨーロッパウナギ(Anguilla anguilla)と魚類群集構造の検出を提供する	Griffiths et al.
150	Technological advances in biodiversity monitoring: applicability, opportunities and challenges	生物多様性モニタリングの技術的進歩：適用可能性、機会と課題	Stephenson
151	Aquatic suspended particulate matter as source of eDNA for fish metabarcoding	魚類のメタバーコーディングのための環境DNAの供給源としての水生浮遊粒子状物質	Diaz et al.
152	Robotic environmental DNA bio-surveillance of freshwater health	淡水の健康状態のロボテックな環境DNAバイオサーベイランス	Sepulveda et al.
153	Environmental DNA is effective in detecting the federally threatened Louisiana Pinesnake (Pituophis ruthveni)	環境DNAは連邦の脅威にさらされているルイジアナ・パインズネーク (Pituophis ruthveni) の検出に効果的である	Katz et al.
154	Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown‐of‐thorns sea star (Acanthaster cf. solaris) detection	ラテラルフローアッセイ（ディップスティック）による高感度環境DNA検出(Acanthaster cf. solaris)の事例研究	Doytle and Uthicke
155	Thermal stratification and fish thermal preference explain vertical eDNA distributions in lakes	熱成層化と魚類の温度選好性が湖沼における垂直方向の環境DNAの分布を明らかにする	Littlefair et al.
156	Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding	環境DNAメタバーコーディングを用いた熱帯魚類群集モニタリングにおける時空間的な変動	Sales et al.
157	Environmental DNA metabarcoding reveals the presence of a small, quick-moving, nocturnal water shrew in a forest stream	環境 DNA メタバーコーディングにより明らかになった小型で素早い夜行性哺乳類カワネズミの渓流における生息	Yonezawa et al.
158	Evaluating environmental DNA as a tool for detecting an amphibian pathogen using an optimized extraction method	最適抽出法を用いた両生類病原体検出ツールとしての環境DNAの評価	Brannely et al.
159	Environmental DNA analysis provides an overview of distribution patterns of two dojo loach species within the Naka-ikemi Wetland, Fukui Prefecture, Japan	環境DNA解析により、福井県中池見湿地内の2種のドジョウの分布パターンの概要が明らかになった	Okada et al.
160	New eDNA based tool applied to the specific detection and monitoring of the endangered European eel	絶滅の危機に瀕しているヨーロッパウナギの特異的な検出とモニタリングに適用される新しい環境DNAベースのツール	Cardas et al.
161	MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples	MiFishメタバーコーディング：環境DNAおよびその他のサンプルから複数の魚種を同時に検出するためのハイスループットなアプローチ	Miya et al.
162	A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish	魚類の環境DNAメタバーコーディングのためのプライマーの包括的・比較評価	Zhang et al.
163	How to design optimal eDNA sampling strategies for biomonitoring in river networks	河川ネットワークにおけるバイオモニタリングのための最適な環境DNAサンプリング戦略の設計方法	Carraro et al.
164	Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal	環境DNAは大規模なダムの撤去後に回遊魚を追跡するための有効なツールである	Duda et al.
165	Using environmental DNA for biomonitoring of freshwater fish communities: Comparison with established gillnet surveys in a boreal hydroelectric impoundment	淡水魚群集のバイオモニタリングに環境DNAを利用: 北極海の水力発電貯水池における確立された刺し網調査との比較	Boivin-Delisle et al.
166	Application of eDNA metabarcoding in a fragmented lowland river: Spatial and methodological comparison of fish species composition	分断された低地河川における環境DNAメタバーコーディングの応用: 魚種組成の空間的・方法論的比較	Antognazza et al.
167	Development and validation of four environmental DNA assays for species of conservation concern in the South-Central United States	米国中南部における保全が懸念される種のための4つの環境DNAアッセイの開発と検証	Siler et al.
168	Comparison of markers for the monitoring of freshwater benthic biodiversity through DNA metabarcoding	DNAメタバーコーディングによる淡水底生生物の生物多様性モニタリングのためのマーカーの比較	Ficetola et al.
169	Multi‐species models reveal that eDNA metabarcoding is more sensitive than backpack electrofishing for conducting fish surveys in freshwater streams	多種モデルを用いた環境DNAメタバーコーディングは電気漁法よりも淡水域の魚類調査の感度が高いことを解明	McColl-Gausden et al.
170	Environmental (e)DNA: what’s behind the term? Clarifying the terminology and recommendations for its future use in biomonitoring	環境(e)DNA：用語の背景にあるものとは？用語の明確化と今後のバイオモニタリングでの使用のための推奨事項	Pawlowski et al.
171	Marine biomonitoring with eDNA: can metabarcoding of water samples cut it as a tool for surveying benthic communities?	環境DNAを用いた海洋バイオモニタリング: 水試料のメタバーコーディングは底生生物群集の調査ツールとして利用できるか？	Antich et al.
172	Increasing confidence for discerning species and population compositions from metabarcoding assays of environmental samples: case studies of fishes in the Laurentian Great Lakes and Wabash River	環境試料のメタバーコーディングアッセイから種と個体群組成を識別するための信頼性の向上：ローレンシャン五大湖とワバッシュ川の魚類の事例研究	Snyer et al.
173	Certain detection of uncertain taxa: eDNA detection of a cryptic mountain sucker (Pantosteus jordani) in the Upper Missouri River, USA	正確な検出による不明な分類群の検出: アメリカ ミズーリ川上流に生息する Pantosteus jordaniの環境DNAの検出	Mason et al.
174	Integrating species distribution and occupancy modeling to study hellbender (Cryptobranchus alleganiensis) occurrence based on eDNA surveys	環境DNA調査に基づいたヘルベンダー(Cryptobranchus alleganiensis)の生息調査のための種の分布と占有モデルの統合	Da silva Neto et al.
175	Disease in Central Valley Salmon: Status and Lessons from Other Systems	セントラルバレーにおけるサケの病気: 他のシステムの現状と教訓	Lehman et al.
176	A non-lethal method for detection of Bonamia ostreae in flat oyster (Ostrea edulis) using environmental DNA	環境DNAによるカキOstrea edulisの寄生虫Bonamia ostreaeの非致死的な検出法	Jorgensen et al.
177	Application of Environmental DNA for Monitoring of Freshwater Fish in Korea	環境DNAの韓国淡水魚モニタリングへの応用	Jeong-Hui et al.
178	Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections	LAMP法を用いた吸虫感染症の制御のための放牧地の水源からGalba truncatulaの迅速的な検出	Davis et al.
179	Assessing the impact of the threatened crucian carp (Carassius carassius) on pond invertebrate diversity ‐ a comparison of conventional and molecular tools	絶滅の危機に瀕しているフナ(Carassius carassius)が池の無脊椎動物の多様性に与える影響の評価-従来のツールと分子ツールの比較	Harper et al.
180	Comparing environmental DNA metabarcoding and underwater visual census to monitor tropical reef fishes	環境DNAメタバーコーディングと水中目視調査の比較による熱帯サンゴ礁魚類のモニタリング	Fernández et al.
181	Environmental DNA shedding and decay rates from diverse animal forms and thermal regimes	多様な動物の形態と熱環境からの環境DNAの脱落と崩壊速度	Allan et al.
182	Mitochondrial genomes of Danish vertebrate species generated for the national DNA reference database, DNAmark	デンマークの脊椎動物種のミトコンドリアゲノムは、国のDNAリファレンスデータベースであるDNAmarkのために作成された。	Margaryan et al.
183	Sedimentary DNA tracks decadal-centennial changes in fish abundance	堆積DNAは魚類資源の10年ごとの変化を追跡する	Kuwae et al.
184	Evaluation of fish biodiversity in estuaries using environmental DNA metabarcoding	環境DNAメタバーコーディングを用いた河口域における魚類の生物多様性の評価	Ahn et al.
185	Comparing fish prey diversity for a critically endangered aquatic mammal in a reserve and the wild using eDNA metabarcoding	環境DNAメタバーコーディングを用いた絶滅危惧種の水生哺乳類の餌生物多様性の野生と保護区の比較	Qu et al.
186	Broad-scale detection of environmental DNA for an invasive macrophyte and the relationship between DNA concentration and coverage in rivers	河川内における侵略的な大型植物の環境DNAの広域的な検出に関するDNA濃度とカバレッジの関係	Doi et al.
187	Population differentiation from environmental DNA: Investigating the potential of haplotype presence/absence‐based analysis of molecular variance	環境DNAからの集団分化：ハプロタイプの有無に基づく分子分散解析の可能性を探る	Azarian et al.
188	Using environmental DNA and occupancy modeling to estimate rangewide metapopulation dynamics	環境DNAと占有モデルを用いた広域的なメタ個体群動態の推定	Martel et al.
189	Benthic monitoring of oil and gas offshore platforms in the North Sea using environmental DNA metabarcoding	環境DNAメタバーコーディングを用いた北海石油・ガス洋上プラットフォームの底生モニタリング	Mauffrey et al.
190	Exploring a legendary giant squid: an environmental DNA approach	環境DNAによるダイオウイカの探索	Doi et al.
191	Environmental DNA Monitoring: Better Tracking of Endangered, Rare, Cryptic, and Invasive Species	環境DNAモニタリング: 絶滅の危機に瀕している種、希少種、隠ぺい種、侵略種のよりよい追跡法	Ota et al.
192	Fish and crustacean biodiversity in an outer maritime estuary of the Pearl River Delta revealed by environmental DNA	環境DNAによる珠江デルタ外洋河口域における魚類・甲殻類の生物多様性の解明	Cheang et al.
193	Identifying spawning events in fish by observing a spike in environmental DNA concentration after spawning	産卵後の環境DNA濃度の急上昇を観察することで、魚の産卵イベントを特定する	Tsuji et al.
194	Allometric scaling of eDNA production in stream‐dwelling brook trout (Salvelinus fontinalis) inferred from population size structure	母集団サイズ構造から推定される渓流域に生息するカワマス（Salvelinus fontinalis）における環境DNA産生のアロメトリック・スケーリング	Yates et al.

環境DNA論文サマリーシート(~2019年)

2019年までの環境DNA論文に関するサマリーシートです。さすがに500報を訳すのはしんどいので、こちらには日本語訳がありません。

最終更新: 2020/10/5
情報元: Tsuji et al.2019

No.	Title	Author	Year
1	Species detection using environmental DNA from water samples	Ficetola et al.	2008
2	From molecules to management: Adopting DNA-based methods for monitoring biological invasions in aquatic environments	Darling and Mahon	2011
3	Persistence of Environmental DNA in Freshwater Ecosystems	Dejean et al.	2011
4	Molecular Detection of Vertebrates in Stream Water: A Demonstration Using Rocky Mountain Tailed Frogs and Idaho Giant Salamanders	Goldberg et al.	2011
5	“Sight-unseen” detection of rare aquatic species using environmental DNA	Jerde et al.	2011
6	Molecular Detection of Invasive Species in Heterogeneous Mixtures Using a Microfluidic Carbon Nanotube Platform	Mahon et al.	2011
7	Meta-barcoding of ‘dirt’ DNA from soil reflects vertebrate biodiversity	Andersen et al.	2012
8	The use of molecular tools in invasion biology: an emphasis on freshwater ecosystems	Blanchet	2012
9	Improved detection of an alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus	Dejean et al.	2012
10	Investigating the Potential Use of Environmental DNA (eDNA) for Genetic Monitoring of Marine Mammals	Foote et al.	2012
11	Response to Casey et al.’s sensitivity of detecting environmental DNA comment	Jerde et al.	2012
12	Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNA	Lodge et al.	2012
13	Surveillance of fish species composition using environmental DNA	Minamoto et al.	2012
14	An eDNA approach to detect eastern hellbenders (Cryptobranchus a. alleganiensis) using samples of water	Olson et al.	2012
15	Next-generation sequencing technologies for environmental DNA research	Shokralla et al.	2012
16	Environmental DNA	Taberlet et al.	2012
17	Estimation of Fish Biomass Using Environmental DNA	Takahara et al.	2012
18	Detection of a Diverse Marine Fish Fauna Using Environmental DNA from Seawater Samples	Thomsen et al.	2012
19	Monitoring endangered freshwater biodiversity using environmental DNA	Thomsen et al.	2012
20	The future of environmental DNA in ecology	Yoccoz	2012
21	Combining species-specific COI primers with environmental DNA analysis for targeted detection of rare freshwater species	Bronnenhuber and Wilson	2013
22	Something in the water: biosecurity monitoring of ornamental fish imports using environmental DNA	Collins et al.	2013
23	Rapid invasive species detection by combining environmental DNA with Light Transmission Spectroscopy	Egan et al.	2013
24	Environmental DNA as a new method for early detection of New Zealand mudsnails (Potamopyrgus antipodarum)	Goldberg et al.	2013
25	Detection of Asian carp DNA as part of a Great Lakes basin-wide surveillance program	Jerde et al.	2013
26	Validation of eDNA Surveillance Sensitivity for Detection of Asian Carps in Controlled and Field Experiments	Mahon et al.	2013
27	Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples	Pilliod et al.	2013
28	Noninvasive Method for a Statewide Survey of Eastern Hellbenders Cryptobranchus alleganiensis Using Environmental DNA	Santas et al.	2013
29	Using Environmental DNA to Estimate the Distribution of an Invasive Fish Species in Ponds	Takahara et al.	2013
30	Robust Detection of Rare Species Using Environmental DNA: The Importance of Primer Specificity	Wilcox et al.	2013
31	Environmental Conditions Influence eDNA Persistence in Aquatic Systems	Barnes et al.	2014
32	Environmental DNA for wildlife biology and biodiversity monitoring	Bohmann et al.	2014
33	Transport Distance of Invertebrate Environmental DNA in a Natural River	Deiner and Altermatt	2014
34	History, applications, methodological issues and perspectives for the use of environmental DNA (eDNA) in marine and freshwater environments	Díaz-Ferguson and Moyer	2014
35	Development of molecular markers for eDNA detection of the invasive African jewelfish (Hemichromis letourneuxi): a new tool for monitoring aquatic invasive species in National Wildlife Refuges	Díaz-Ferguson et al.	2014
36	The Relationship between the Distribution of Common Carp and Their Environmental DNA in a Small Lake	Eichmiller et al.	2014
37	Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data	Ficetola et al.	2014
38	Distance, flow and PCR inhibition: eDNA dynamics in two headwater streams	Jane et al.	2014
39	Using Environmental DNA to Census Marine Fishes in a Large Mesocosm	Kelly et al.	2014
40	Detection of invasive freshwater fish species using environmental DNA survey	Keskin	2014
41	Utility of environmental DNA for monitoring rare and indicator macroinvertebrate species	Mächler et al.	2014
42	Meta-genomic surveillance of invasive species in the bait trade	Mahon et al.	2014
43	The Release Rate of Environmental DNA from Juvenile and Adult Fish	Maruyama et al.	2014
44	Persistence of DNA in Carcasses, Slime and Avian Feces May Affect Interpretation of Environmental DNA Data	Merkes et al.	2014
45	Assessing Environmental DNA Detection in Controlled Lentic Systems	Moyer et al.	2014
46	Quantifying Environmental DNA Signals for Aquatic Invasive Species Across Multiple Detection Platforms	Nathan et al.	2014
47	The Use of Environmental DNA in Invasive Species Surveillance of the Great Lakes Commercial Bait Trade	Nathan et al.	2014
48	Detecting an elusive invasive species: a diagnostic PCR to detect Burmese python in Florida waters and an assessment of persistence of environmental DNA	Piaggio et al.	2014
49	Factors influencing detection of eDNA from a stream-dwelling amphibian	Pilliod et al.	2014
50	The application of eDNA for monitoring of the Great Crested Newt in the UK	Rees et al.	2014
51	The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology	Rees et al.	2014
52	Environmental DNA surveillance for invertebrate species: advantages and technical limitations to detect invasive crayfish Procambarus clarkii in freshwater ponds	Tréguier et al.	2014
53	Particle size distribution and optimal capture of aqueous macrobial eDNA	Turner et al.	2014
54	Improved Methods for Capture, Extraction, and Quantitative Assay of Environmental DNA from Asian Bigheaded Carp (Hypophthalmichthys spp.)	Turner et al.	2014
55	Tracking ghosts: combined electrofishing and environmental DNA surveillance efforts for Asian carps in Ontario waters of Lake Erie	Wilson et al.	2014
56	Improving efficiency and reliability of environmental DNA analysis for silver carp	Amberg et al.	2015
57	Environmental DNA evidence of transfer of North Sea molluscs across tropical waters through ballast water	Ardura et al.	2015
58	eDNA and specific primers for early detection of invasive species e A case study on the bivalve Rangia cuneata, currently spreading in Europe	Ardura et al.	2015
59	Diverse Applications of Environmental DNA Methods in Parasitology	Bass et al.	2015
60	Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus)	Biggs et al.	2015
61	Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles	Davy et al.	2015
62	Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA	Deiner et al.	2015
63	Droplet Digital Polymerase Chain Reaction (PCR) Outperforms Real-Time PCR in the Detection of Environmental DNA from an Invasive Fish Species	Doi et al.	2015
64	Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass inEnvironmental DNA Surveys	Doi et al.	2015
65	Rapid molecular detection of invasive species in ballast and harbor water by integrating environmental DNA and Light Transmission Spectroscopy	Egan et al.	2015
66	Mitochondrial Genome Sequencing and Development of Genetic Markers for the Detection of DNA of Invasive Bighead and Silver Carp (Hypophthalmichthys nobilis and H. molitrix) in Environmental Water Samples from the United States	Farrington et al.	2015
67	A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan	Fukumoto et al.	2015
68	A framework for estimating the sensitivity of eDNA surveys	Furlan et al.	2015
69	Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms	Goldberg et al.	2015
70	An eDNA assay for Irish Petromyzon marinus and Salmo trutta and field validation in running water	Gustavson et al.	2015
71	How will the ‘molecular revolution’ contribute to biological recording?	Handley et al.	2015
72	Environmental DNA (eDNA) Sampling Improves Occurrence and Detection Estimates of Invasive Burmese Pythons	Hunter et al.	2015
73	Development and application of an eDNA method to detect and quantify a pathogenic parasite in aquatic ecosystems	Huver et al.	2015
74	Environmental DNA as an effective tool for detection of imperiled fishes	Janosik and Johnston	2015
75	Improving confidence in environmental DNA species detection	Jerde et al.	2015
76	Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix	Klymus et al.	2015
77	A reply to Iversen et al.’s comment “Monitoring of animal abundance by environmental DNA — An increasingly obscure perspective”	Klymus et al.	2015
78	Characterizing the distribution of an endangered salmonid using environmental DNA analysis	Laramie et al.	2015
79	Characterizing the distribution of an endangered salmonid using environmental DNA analysis	Laramie et al.	2015
80	Monitoring of animal abundance by environmental DNA — An increasingly obscure perspective: A reply to Klymus et al., 2015	Lversen et al.	2015
81	The effect of dilution and the use of a post-extraction nucleic acid purification column on the accuracy, precision, and inhibition of environmental DNA samples	McKee et al.	2015
82	Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction	Minamoto et al.	2015
83	MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fshes: detection of more than 230subtropical marine species	Miya et al.	2015
84	Reconstructing long-term human impacts on plant communities: an ecological approach based on lake sediment DNA	Pansu et al.	2015
85	Ancient and modern environmental DNA	Pedersen et al.	2015
86	Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals	Rees et al.	2015
87	The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol–chloroform–isoamyl alcohol DNA extraction	Renshaw et al.	2015
88	Drinking water as a source of environmental DNA for the detection of terrestrial wildlife species	Rodgers and Mock	2015
89	The downside of eDNA as a survey tool in water bodies	Roussel et al.	2015
90	Modeling the Sensitivity of Field Surveys for Detection of Environmental DNA (eDNA)	Schultz and Lance	2015
91	Development of species-specific environmental DNA (eDNA) markers for invasive aquatic plants	Scriver et al.	2015
92	Monitoring the near-extinct European weather loach in Denmark based on environmental DNA from water samples	Sigsgaard et al.	2015
93	Active and passive environmental DNA surveillance of aquatic invasive species	Simmons et al.	2015
94	Environmental DNA sampling is more sensitive than a traditional survey technique for detecting an aquatic invader	Smart et al.	2015
95	Using environmental DNA methods to improve detectability in a hellbender (Cryptobranchus alleganiensis) monitoring program	Spear et al.	2015
96	Quantifying effects of UV-B, temperature, and pH on eDNA degradation in aquatic microcosms	Strickler et al.	2015
97	Effects of sample processing on the detection rate of environmental DNA from the Common Carp (Cyprinus carpio)	Takahara et al.	2015
98	Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity	Thomsen et al.	2015
99	Fish environmental DNA is more concentrated in aquatic sediments than surface water	Turner et al.	2015
100	Long duration, room temperature preservation of filtered eDNA samples	Wegleitner et al.	2015
101	Environmental DNA particle size distribution from Brook Trout (Salvelinus fontinalis)	Wilcox et al.	2015
102	The Dual Challenges of Generality and Specificity When Developing Environmental DNA Markers for Species and Subspecies of Oncorhynchus	Wilcox et al.	2015
103	An eDNA Assay to Monitor a Globally Invasive Fish Species from Flowing Freshwater	Adrian-Kalchhauser and Burkhardt-Holm	2016
104	The ecology of environmental DNA and implications for conservation genetics	Barnes and Turner	2016
105	Detection of Adult Green Sturgeon Using Environmental DNA Analysis	Bergman et al.	2016
106	Environmental DNA (eDNA) detection and habitat occupancy of threatened spotted gar (Lepisosteus oculatus)	Boothroyd et al.	2016
107	A primer for use of genetic tools in selecting and testing the suitability of set-aside sites protected from deep-sea seafloor massive sulfide mining activities	Boschen et al.	2016
108	Metabarcoding of marine zooplankton: prospects, progress and pitfalls	Bucklin et al.	2016
109	Improving the containment of a freshwater invader using environmental DNA (eDNA) based monitoring	Bylemans et al.	2016
110	In silico assessment of primers for eDNA studies using PrimerTree and application to characterize the biodiversity surrounding the Cuyahoga River	Cannon et al.	2016
111	Environmental DNA Marker Development with Sparse Biological Information: A Case Study on Opossum Shrimp (Mysis diluviana)	Carim et al.	2016
112	An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America	Carim et al.	2016
113	An environmental DNA marker for detecting nonnative brown trout (Salmo trutta)	Carim et al.	2016
114	Development of species-specific primers with potential for amplifying eDNA from imperilled freshwater unionid mussels	Cho et al.	2016
115	Spatial Representativeness of Environmental DNA Metabarcoding Signal for Fish Biodiversity Assessment in a Natural Freshwater System	Civade et al.	2016
116	An Easy Phylogenetically Informative Method to Trace the Globally Invasive Potamopyrgus Mud Snail from River’s eDNA	Clusa et al.	2016
117	Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA	Davison et al.	2016
118	Detection and characterisation of the biopollutant Xenostrobus securis (Lamarck 1819) Asturian population from DNA Barcoding and eBarcoding	Devloo-Delva et al.	2016
119	Environmental DNA for Detection of Endangered Grouper Species (Epinephelus spp.)	Doğdu and Turan	2016
120	Environmental DNA analysis for estimating the abundance and biomass of stream fish	Doi et al.	2016
121	Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances	Dougherty et al.	2016
122	Potential of EnvironmentalDNA to Evaluate Northern Pike (Esox lucius) Eradication Efforts: An Experimental Test and Case Study	Dunker et al.	2016
123	Quantitative PCR Assays for Detecting Loach Minnow (Rhinichthys cobitis) and Spikedace (Meda fulgida) in the Southwestern United States	Dysthe et al.	2016
124	Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish	Eichmiller et al.	2016
125	Effects of temperature and trophic state on degradation of environmental DNA in lake water	Eichmiller et al.	2016
126	Detecting the movement and spawning activity of bigheaded carps with environmental DNA	Erickson et al.	2016
127	Trails of river monsters: Detecting critically endangered Mekong giant catfish Pangasianodon gigas using environmental DNA	Eva et al.	2016
128	Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding	Evans et al.	2016
129	Large-Scale Monitoring of Plants through Environmental DNA Metabarcoding of Soil: Recovery, Resolution, and Annotation of Four DNA Markers	Fahner et al.	2016
130	How to limit false positives in environmental DNA and metabarcoding?	Ficetola et al.	2016
131	Can environmental DNA (eDNA) be used for detection and monitoring of introduced crab species in the Baltic Sea?	Forsström and Vasemägi	2016
132	The importance of molecular markers and primer design when characterizing biodiversity from environmental DNA (eDNA)	Freeland	2016
133	Use of environmental DNA to survey the distribution of an invasive submerged plant in ponds	Fujiwara et al.	2016
134	Environmental DNA detection of redfin perch, Perca fluviatilis	Furlan and Gleeson	2016
135	Detection and identification of lampreys in Great Lakes streams using environmental DNA	Gingera et al.	2016
136	Critical considerations for the application of environmental DNA methods to detect aquatic species	Goldberg et al.	2016
137	Environmental DNA metabarcoding of lake fish communities reflects long-term data from established survey methods	Hänfling et al.	2016
138	Aquatic biodiversity assessment for the lazy	Hoffmann et al.	2016
139	Using environmental DNA to detect an endangered crayfish Cambaroides japonicus in streams	Ikeda et al.	2016
140	The influence of stream bottom substrate on the retention and transport of vertebrate environmental DNA	Jerde et al.	2016
141	Making environmental DNA count	Kelly	2016
142	Genetic signatures of ecological diversity along an urbanization gradient	Kelly et al.	2016
143	Detection of rare and invasive freshwater fish species using eDNA pyrosequencing: Lake Iznik ichthyofauna revised	Keskin et al.	2016
144	Quantifying relative fish abundance with eDNA: a promising tool for fisheries management	Lacoursière-Roussel et al.	2016
145	Estimating fish abundance and biomass from eDNA concentrations: variability among capture methods and environmental conditions	Lacoursière-Roussel et al.	2016
146	Statistical approaches to account for false-positive errors in environmental DNA samples	Lahoz-Monfort et al.	2016
147	Characterization, optimization, and validation of environmental DNA (eDNA) markers to detect an endangered aquatic mammal	Ma et al.	2016
148	Fishing in the Water: Effect of Sampled Water Volume on Environmental DNA-Based Detection of Macroinvertebrates	Mächler et al.	2016
149	Evaluation of the Environmental DNA Method for Estimating Distribution and Biomass of Submerged Aquatic Plants	Matsuhashi et al.	2016
150	Sampling large geographic areas for rare species using environmental DNA: a study of bull trout Salvelinus confluentus occupancy in western Montana	Mckelvey et al.	2016
151	Controlling populations of invasive pygmy mussel (Xenostrobus securis) through citizen science and environmental DNA	Miralles et al.	2016
152	Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA	Miya et al.	2016
153	Estimating species richness using environmental DNA	Olds et al.	2016
154	An eDNA assay for river otter detection: a tool for surveying a semi-aquatic mammal	Padgett-Stewart et al.	2016
155	Saving the doomed: Using eDNA to aid in detection of rare sturgeon for conservation (Acipenseridae)	Pfleger et al.	2016
156	Evaluating the effects of laboratory protocols on eDNA detection probability for an endangered freshwater fish	Piggott	2016
157	Assessing Vertebrate Biodiversity in a Kelp Forest Ecosystem using Environmental DNA	Port et al.	2016
158	Fine tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems	Robson et al.	2016
159	Improving herpetological surveys in eastern North America using the environmental DNA method	Roussel et al.	2016
160	Quantification of environmental DNA (eDNA) shedding and decay rates for three marine fish	Sassoubre et al.	2016
161	Using occupancy modelling to compare environmental DNA to traditional field methods for regional-scale monitoring of an endangered aquatic species	Schmelzle and Inziger	2016
162	Detection of Invasive Mosquito Vectors Using Environmental DNA (eDNA) from Water Samples	Schneider et al.	2016
163	Detection of a global aquatic invasive amphibian, Xenopus laevis, using environmental DNA	Secondi et al.	2016
164	Comparison of environmental DNA metabarcoding and conventional fish survey methods in a river system	Shaw et al.	2016
165	A framework for inferring biological communities from environmental DNA	Shelton et al.	2016
166	Modelling the transport of environmental DNA through a porous substrate using continuous flow-through column experiments	Shogren et al.	2016
167	Population characteristics of a large whale shark aggregation inferred from seawater environmental DNA	Sigsgaard et al.	2016
168	Environmental DNA detects Critically Endangered largetooth sawfish in the wild	Simpfendorfer et al.	2016
169	Worldwide analysis of sedimentary DNA reveals major gaps in taxonomic knowledge of deep-sea benthos	Sinniger et al.	2016
170	Assessing the cost-efficiency of environmental DNA sampling	Smart et al.	2016
171	Inferring neutral biodiversity parameters using environmental DNA data sets	Sommeria-Klein et al.	2016
172	Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms	Souza et al.	2016
173	Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter	Spens et al.	2016
174	Environmental DNA as a monitoring tool for the endangered freshwater pearl mussel (Margaritifera margaritifera L.): a substitute for classical monitoring approaches?	Stoeckle et al.	2016
175	Emerging Technologies to Conserve Biodiversity: Further Opportunities via Genomics. Response to Pimm et al.	Taylor et al.	2016
176	Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes	Thomsen et al.	2016
177	Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples	Tsuji et al.	2016
178	A sensitive environmental DNA (eDNA) assay leads to new insights on Ruffe (Gymnocephalus cernua) spread in North America	Tucker et al.	2016
179	A novel environmental DNA approach to quantify the cryptic invasion of non-native genotypes	Uchii et al.	2016
180	Next-generation monitoring of aquatic biodiversity using environmental DNA metabarcoding	Valentini et al.	2016
181	Implementation of Novel Design Features for qPCR-Based eDNA Assessment	Veldhoen et al.	2016
182	Freshwater vertebrate metabarcoding on Illumina platforms using double-indexed primers of the mitochondrial 16S rRNA gene	Vences et al.	2016
183	Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis	Wilcox et al.	2016
184	No filter, no fridges: a method for preservation of water samples for eDNA analysis	Williams et al.	2016
185	The importance of including imperfect detection models in eDNA experimental design	Willoughby et al.	2016
186	Recognizing false positives: synthetic oligonucleotide controls for environmental DNA surveillance	Wilson et al.	2016
187	Environmental DNA as a ‘Snapshot’ of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan	Yamamoto et al.	2016
188	The use of environmental DNA of fishes as an efficient method of determining habitat connectivity	Yamanaka and Minamoto	2016
189	On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation	Yamanaka et al.	2016
190	Monitoring of noble, signal and narrowclawed crayfish using environmental DNA from freshwater samples	Agersnap et al.	2017
191	Biomonitoring of marine vertebrates in Monterey Bay using eDNA metabarcoding	Andruszkiewicz et al.	2017
192	Persistence of marine fish environmental DNA and the influence of sunlight	Andruszkiewicz et al.	2017
193	Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact	Bakker et al.	2017
194	Residual eDNA detection sensitivity assessed by quantitative real-time PCR in a river ecosystem	Balasingham et al.	2017
195	Efficacy of Environmental DNA to Detect and Quantify Brook Trout Populations in Headwater Streams of the Adirondack Mountains, New York	Baldigo et al.	2017
196	Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity	Bista et al.	2017
197	Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples – first record of Gammarus fossarum in the UK	Blackman et al.	2017
198	DNA in a bottle—Rapid metabarcoding survey for early alerts of invasive species in ports	Borrell et al.	2017
199	Development and Application of an eDNA Method to Detect the Critically Endangered Trinidad Golden Tree Frog (Phytotriades auratus) in Bromeliad Phytotelmata	Brozio et al.	2017
200	Is the detection of aquatic environmental DNA influenced by substrate type?	Buxton et al.	2017
201	Seasonal variation in environmental DNA in relation to population size and environmental factors	Buxton et al.	2017
202	An environmental DNA-based method for monitoring spawning activity: a case study, using the endangered Macquarie perch (Macquaria australasica)	Bylemans et al.	2017
203	Using eDNA to Detect the Distribution and Density of Invasive Crayfish in the Honghe-Hani Rice Terrace World Heritage Site	Cai et al.	2017
204	A Noninvasive Tool to Assess the Distribution of Pacific Lamprey (Entosphenus tridentatus) in the Columbia River Basin	Carim et al.	2017
205	A qPCR MGB probe based eDNA assay for European freshwater pearl mussel (Margaritifera margaritifera L.)	Carlsson et al.	2017
206	eDNA for detection of five highly invasive molluscs. A case study in urban rivers from the Iberian Peninsula	Clusa et al.	2017
207	An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples	Clusa et al.	2017
208	Predicting the ecological quality status of marine environments from eDNA metabarcoding data using supervised machine learning	Cordier et al.	2017
209	Application of environmental DNA analysis to inform invasive fish eradication operations	Davison et al.	2017
210	Environmental DNA metabarcoding: Transforming how we survey animal and plant communities	Deiner et al.	2017
211	Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA	Deiner et al.	2017
212	Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels	Djurhuus et al.	2017
213	Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem	Doi et al.	2017
214	Isopropanol precipitation method for collecting fish environmental DNA	Doi et al.	2017
215	Water sampling for environmental DNA surveys by using an unmanned aerial vehicle	Doi et al.	2017
216	Behavior and season affect crayfish detection and density inference using environmental DNA	Dunn et al.	2017
217	Environmental DNA assays for the sister taxa sauger (Sander canadensis) and walleye (Sander vitreus)	Dysthe et al.	2017
218	Seasonal trends in eDNA detection and occupancy of bigheaded carps	Erickson et al.	2017
219	Fish community assessment with eDNA metabarcoding: effects of sampling design and bioinformatic filtering	Evans et al.	2017
220	Comparative Cost and Effort of Fish Distribution Detection via Environmental DNA Analysis and Electrofishing	Evans et al.	2017
221	Improving reliability in environmental DNA detection surveys through enhanced quality control	Furlan and Gleeson	2017
222	Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: a case study of devil ray at seamounts	Gargan et al.	2017
223	qPCR Detection of Early Life History Stage Chrysaora quinquecirrha (Sea Nettles) in Barnegat Bay, New Jersey	Gaynor et al.	2017
224	Management of invasive species in inland waters: technology development and international cooperation	Gingera et al.	2017
225	Use of environmental DNA (eDNA) and water quality data to predict protozoan parasites outbreaks in fish farms	Gomes et al.	2017
226	Environmental DNA in subterranean biology: range extension and taxonomic implications for Proteus	Gorički et al.	2017
227	Dealing with false positive and false negative errors about species occurrence at multiple levels	Guillera-Arroita et al.	2017
228	Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples	Hashizume et al.	2017
229	Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting	Hinlo et al.	2017
230	Methods to maximise recovery of environmental DNA from water samples	Hinlo et al.	2017
231	Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA	Hunter et al.	2017
232	Tropical-forest mammals as detected by environmental DNA at natural saltlicks in Borneo	Ishige et al.	2017
233	Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA	Jo et al.	2017
234	Environmental DNA method for estimating salamander distribution in headwater streams, and a comparison of water sampling methods	Katano et al.	2017
235	Genetic and Manual Survey Methods Yield Different and Complementary Views of an Ecosystem	Kelly et al.	2017
236	At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations	Klobucar et al.	2017
237	Metabarcoding of Environmental DNA Samples to Explore the Use of Uranium Mine Containment Ponds as a Water Source for Wildlife	Klymus et al.	2017
238	Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes	Klymus et al.	2017
239	Experimental observations on the decay of environmental DNA from bighead and silver carps	Lance et al.	2017
240	Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America	Larson et al.	2017
241	DNA amplification in the field: move over PCR, here comes LAMP	Lee et al.	2017
242	eDNA metabarcoding: a promising method for anuran surveys in highly diverse tropical forests	Lopes et al.	2017
243	A framework for developing and validating taxon‐specific primers for specimen identification from environmental DNA	MacDonald and Sarre	2017
244	A Rapid‐Assessment Method to Estimate the Distribution of Juvenile Chinook Salmon in Tributary Habitats Using eDNA and Occupancy Estimation	Matter et al.	2017
245	On the way for detecting and quantifying elusive species in the sea: The Octopus vulgaris case study	Mauvisseau et al.	2017
246	Environmental DNA reflects spatial and temporal jellyfish distribution	Minamoto et al.	2017
247	Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio	Minamoto et al.	2017
248	Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid	Mizumoto et al.	2017
249	Using Environmental DNA to Improve Species Distribution Models for Freshwater Invaders	Muha et al.	2017
250	Spatial distribution of environmental DNA in a nearshore marine habitat	O’Donnell et al.	2017
251	Comparison of American Fisheries Society (AFS) Standard Fish Sampling Techniques and Environmental DNA for Characterizing Fish Communities in a Large Reservoir	Perez et al.	2017
252	An environmental DNA assay for detecting Macquarie perch, Macquaria australasica	Piggott	2017
253	Decline of a giant salamander assessed with historical records, environmental DNA and multi‐scale habitat data	Pitt et al.	2017
254	Wanted dead or alive? Using metabarcoding of environmental DNA and RNA to distinguish living assemblages for biosecurity applications	Pochon et al.	2017
255	The detection of great crested newts year round via environmental DNA analysis	Rees et al.	2017
256	Living quarters of a living fossil – Uncovering the current distribution pattern of the rediscovered Hula painted frog (Latonia nigriventer) using environmental DNA	Renan et al.	2017
257	Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan	Sakata et al.	2017
258	Environmental DNA (eDNA) Shedding and Decay Rates to Model Freshwater Mussel eDNA Transport in a River	Sansom et al.	2017
259	Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna	Sasso et al.	2017
260	Usefulness and limitations of sample pooling for environmental DNA metabarcoding of freshwater fsh communities	Sato et al.	2017
261	Controls on eDNA movement in streams: Transport, Retention, and Resuspension	Shogren et al.	2017
262	Seawater environmental DNA refects seasonality of a coastal fsh community	Sigsgaard et al.	2017
263	Making sense of the noise: The effect of hydrology on silver carp eDNA detection in the Chicago area waterway system	Song et al.	2017
264	Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA	Spangler et al.	2017
265	Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment	Stat et al.	2017
266	Using environmental DNA to assess population-wide spatiotemporal reserve use	Stewart et al.	2017
267	A systematic approach to evaluate the influence of environmental conditions on eDNA detection success in aquatic ecosystems	Stoeckle et al.	2017
268	Aquatic environmental DNA detects seasonal fish abundance and habitat preference in an urban estuary	Stoeckle et al.	2017
269	Exploring the Use of Environmental DNA to Determine the Species of Salmon Redds	Strobel et al.	2017
270	Seasonal and diel signature of eastern hellbender environmental DNA	Takahashi et al.	2017
271	Environmental DNA Detection of the Golden Tree Frog (Phytotriades auratus) in Bromeliads	Torresdal et al.	2017
272	Water temperature-dependent degradation of environmental DNA and its relation to bacterial abundance	Tsuji et al.	2017
273	Distinct seasonal migration patterns of Japanese native and non-native genotypes of common carp estimated by environmental DNA	Uchii et al.	2017
274	Comparing Efficiency of American Fisheries Society Standard Snorkeling Techniques to Environmental DNA Sampling Techniques	Ulibarri et al.	2017
275	Environmental DNA enables detection of terrestrial mammals from forest pond water	Ushio et al.	2017
276	Tracing the quagga mussel invasion along the Rhine river system using eDNA markers: early detection and surveillance of invasive zebra and quagga mussels	Ventura et al.	2017
277	Using DNA metabarcoding to investigate honey bee foraging reveals limited flower use despite high floral availability	Vere et al.	2017
278	Surveying Europe’s Only Cave-Dwelling Chordate Species (Proteus anguinus) Using Environmental DNA	Vörös et al.	2017
279	Methodological considerations for detection of terrestrial small-body salamander eDNA and implications for biodiversity conservation	Walker et al.	2017
280	Application of environmental DNA to detect an endangered marine skate species in the wild	Weltz et al.	2017
281	Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.	Williams et al.	2017
282	Clearing muddied waters: Capture of environmental DNA from turbid waters	Williams et al.	2017
283	Quantitative PCR multiplexes for simultaneous multispecies detection of Asian carp eDNA	Wozney et al.	2017
284	Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea	Yamamoto et al.	2017
285	A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant	Yamanaka et al.	2017
286	Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation	Alsos et al.	2018
287	The Importance of Sound Methodology in Environmental DNA Sampling: Response to Comment	Amberg et al.	2018
288	PCR-based assay for Mya arenaria detection from marine environmental samples and tracking its invasion in coastal ecosystems	Ardura et al.	2018
289	An environmental DNA sampling method for aye-ayes from their feeding traces	Aylward et al.	2018
290	Evaluation of environmental DNA to detect Sistrurus catenatus and Ophidiomyces ophiodiicola in crayfish burrows	Baker et al.	2018
291	Environmental DNA (eDNA) From the Wake of the Whales: Droplet Digital PCR for Detection and Species Identification	Baker et al.	2018
292	Environmental DNA detection of rare and invasive fish species in two Great Lakes tributaries	Balasingham et al.	2018
293	Environmental DNA Time Series in Ecology	Bálint et al.	2018
294	Accuracy, limitations and cost efficiency of eDNA‐based community survey in tropical frogs	Bálint et al.	2018
295	Core Concept: Environmental DNA helps researchers track pythons and other stealthy creatures	Beans	2018
296	Development of quantitative PCR primers and probes for environmental DNA detection of amphibians in Ontario	Beauclerc et al.	2018
297	Metabarcoding and post-sampling strategies to discover non-indigenous species: A case study in the estuaries of the central south Bay of Biscay	Borrell et al.	2018
298	Environmental DNA illuminates the dark diversity of sharks	Boussarie et al.	2018
299	Shotgun metagenomics of honey DNA: Evaluation of a methodological approach to describe a multi-kingdom honey bee derived environmental DNA signature	Bovo et al.	2018
300	Seasonal variation in environmental DNA detection in sediment and water samples	Buxton et al.	2018
301	Comparison of Two Citizen Scientist Methods for Collecting Pond Water Samples for Environmental DNA Studies	Buxton et al.	2018
302	Toward an ecoregion scale evaluation of eDNA metabarcoding primers: A case study for the freshwater fish biodiversity of the Murray–Darling Basin (Australia)	Bylemans et al.	2018
303	Does size matter? An experimental evaluation of the relative abundance and decay rates of aquatic eDNA	Bylemans et al.	2018
304	Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient	Bylemans et al.	2018
305	Estimating species distribution and abundance in river networks using environmental DNA	Carraro et al.	2018
306	A fate and transport model for Asian carp environmental DNA in the Chicago area waterways system	Cerco et al.	2018
307	An analytical framework for estimating aquatic species density from environmental DNA	Chambert et al.	2018
308	Conditionally autoregressive models improve occupancy analyses of autocorrelated data: an example with environmental DNA	Chen and Ficetola	2018
309	Unlocking biodiversity and conservation studies in high diversity environments using environmental DNA (eDNA): a test with Guianese freshwater fishes	Cilleros et al.	2018
310	Persistence of environmental DNA in marine systems	Collins et al.	2018
311	Environmental DNA (eDNA) applications for the conservation of imperiled crayfish (Decapoda: Astacidea) through monitoring of invasive species barriers and relocated populations	Cowart et al.	2018
312	Development and field validation of an environmental DNA (eDNA) assay for invasive clams of the genus Corbicula	Cowart et al.	2018
313	Metagenomic sequencing of environmental DNA reveals marine faunal assemblages from the West Antarctic Peninsula	Cowart et al.	2018
314	Uses and Misuses of Environmental DNA in Biodiversity Science and Conservation	Cristescu and Hebert	2018
315	Validation of environmental DNA (eDNA) as a detection tool for at‐risk freshwater pearly mussel species (Bivalvia: Unionidae)	Currier et al.	2018
316	Accounting for observation processes across multiple levels of uncertainty improves inference of species distributions and guides adaptive sampling of environmental DNA	Davis et al.	2018
317	Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, and extraction method	Deiner et al.	2018
318	Towards robust and repeatable sampling methods in eDNA based studies	Dickie et al.	2018
319	eDNAoccupancy: An R Package for Multi-scale Occupancy Modeling of Environmental DNA Data	Dorazio et al.	2018
320	Repurposing environmental DNA samples—detecting the western pearlshell (Margaritifera falcata) as a proof of concept	Dysthe et al.	2018
321	An improved environmental DNA assay for bull trout (Salvelinus confluentus) based on the ribosomal internal transcribed spacer I	Dysthe et al.	2018
322	Environmental DNA (eDNA) detects the pool frog (Pelophylax lessonae) at times when traditional monitoring methods are insensitive	Eiler et al.	2018
323	Freshwater fisheries assessment using environmental DNA: A primer on the method, its potential, and shortcomings as a conservation tool	Evans et al.	2018
324	Exploring deep-water coral communities using environmental DNA	Everett and Park	2018
325	Environmental DNA for freshwater fish monitoring: insights for conservation within a protected area	Fernandez et al.	2018
326	Evaluating freshwater macroinvertebrates from eDNA metabarcoding: A river Nalo ́n case study	Fernández et al.	2018
327	DNA from lake sediments reveals long-term ecosystem changes after a biological invasion	Ficetola et al.	2018
328	“Pitfalls in relative abundance estimation using eDNA metabarcoding”	Fonseca	2018
329	Inferring presence of the western toad (Anaxyrus boreas) species complex using environmental DNA	Franklin et al.	2018
330	A Non-Invasive Sampling Method for Detecting Non-Native Smallmouth Bass (Micropterus dolomieu)	Franklin et al.	2018
331	Environmental DNA detection of aquatic invasive plants in lab mesocosm and natural field conditions	Gantz et al.	2018
332	A search for standardized protocols to detect alien invasive crayfish based on environmental DNA (eDNA): A lab and field evaluation	Geerts et al.	2018
333	Attracting Common Carp to a bait site with food reveals strong positive relationships between fish density, feeding activity, environmental DNA, and sex pheromone release that could be used in invasive fish management	Ghosal et al.	2018
334	Degradation and dispersion limit environmental DNA detection of rare amphibians in wetlands: Increasing efficacy of sampling designs.	Goldberg et al.	2018
335	Efects of sampling efort on biodiversity patterns estimated from environmental DNA metabarcoding surveys	Grey et al.	2018
336	Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes	Günther et al.	2018
337	Occurrence of California Red-Legged (Rana draytonii) and Northern Red-Legged (Rana aurora) Frogs in Timberlands of Mendocino County, California, Examined with Environmental DNA	Halstead et al.	2018
338	Quantitative real-time polymerase chain reaction (PCR) and droplet digital PCR duplex assays for detecting Zostera marina DNA in coastal sediments digital PCR duplex assays for detecting Zostera marina DNA in coastal sediments	Hamaguchi et al.	2018
339	The sceptical optimist: challenges and perspectives for the application of environmental DNA in marine fisheries	Hansen et al.	2018
340	Development and application of environmental DNA surveillance for the threatened crucian carp (Carassius carassius)	Haper et al.	2018
341	Searching for a signal: Environmental DNA (eDNA) for the detection of invasive signal crayfish, Pacifastacus leniusculus (Dana, 1852)	Harper et al.	2018
342	Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus)	Harper et al.	2018
343	Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds	Harper et al.	2018
344	Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive	Hering et al.	2018
345	Performance of eDNA assays to detect and quantify an elusive benthic fish in upland streams	Hinlo et al.	2018
346	Improving the yield of environmental DNA from filtered aquatic samples	Hundermark and Takahashi	2018
347	Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations	Hunter et al.	2018
348	Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control?	Jones et al.	2018
349	Environmental DNA quantifcation in a spatial and temporal context: a case study examining the removal of brook trout from a high alpine basin	Kamorof and Goldberg	2018
350	An issue of life or death: using eDNA to detect viable individuals in wilderness restoration	Kamoroff et al.	2018
351	Early detection of marine invasive species, Bugula neritina (Bryozoa: Cheilostomatida), using species-specific primers and environmental DNA analysis in Korea.	Kim et al.	2018
352	Environmental DNA as a new tool for assessing the biodiversity of Lake Baikal	Kirilchik	2018
353	Environmental DNA metabarcoding studies are critically affected by substrate selection	Koziol et al.	2018
354	Finding the pond through the weeds: eDNA reveals underestimated diversity of pondweeds	Kuzmina et al.	2018
355	Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA	Ladell et al.	2018
356	Detecting Southern California’s White Sharks With Environmental DNA	Lafferty et al.	2018
357	Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples	Lear et al.	2018
358	Application of Environmental DNA Metabarcoding for Predicting Anthropogenic Pollution in Rivers	Li et al.	2018
359	Estimating fish alpha- and beta-diversity along a small stream with environmental DNA metabarcoding	Li et al.	2018
360	The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding	Li et al.	2018
361	Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities	Lim et al.	2018
362	Optimal survey designs for environmental DNA sampling	Lugg et al.	2018
363	Comparison of environmental DNA and bulk-sample metabarcoding using highly degenerate COI primers	Macher et al.	2018
364	Shedding light on eDNA: neither natural levels of UV radiation nor the presence of a filter feeder affect eDNA-based detection of aquatic organisms	Mächler et al.	2018
365	Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples	Majaneva et al.	2018
366	Environmental DNA fltration techniques afect recovered biodiversit	Majaneva et al.	2018
367	Environmental DNA analysis as a non‐invasive quantitative tool for reproductive migration of a threatened endemic fish in rivers	Maruyama et al.	2018
368	qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples	Mason et al.	2018
369	Environmental DNA as an efficient tool for detecting invasive crayfishes in freshwater ponds	Mauvisseau et al.	2018
370	Using redundant primer sets to detect multiple native Alaskan fish species from environmental DNA	Mennig et al.	2018
371	Comparing local- and regional-scale estimations of the diversity of stream fish using eDNA metabarcoding and conventional observation methods	Nakagawa et al.	2018
372	Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus)	Nevers et al.	2018
373	Evaluation of eDNA for groundwater invertebrate detection and monitoring: a case study with endangered Stygobromus (Amphipoda: Crangonyctidae)	Niemiller et al.	2018
374	Simulating the advection and degradation of the environmental DNA of common carp along a river	Nukazawa et al.	2018
375	Collaborative environmental DNA sampling from petal surfaces of flowering cherry Cerasus×yedoensis ‘Somei-yoshino’ across the Japanese archipelago	Ohta et al.	2018
376	Distribution and seasonal differences in Pacific Lamprey and Lampetra spp eDNA across 18 Puget Sound watersheds	Ostberg et al.	2018
377	Citizen warnings and post checkout molecular confirmations using eDNA as a combined strategy for updating invasive species distributions	Parrondo et al.	2018
378	Water, water everywhere: environmental DNA can unlock population structure in elusive marine species	Parsons et al.	2018
379	The future of biotic indices in the ecogenomic era: Integrating (e)DNA metabarcoding in biological assessment of aquatic ecosystems	Pawlowski et al.	2018
380	Environmental DNA: A New Low-Cost Monitoring Tool for Pathogens in Salmonid Aquaculture	Peters et al.	2018
381	Environmental DNA analysis of river herring in Chesapeake Bay: A powerful tool for monitoring threatened keystone species	Plough et al.	2018
382	Environmental DNA reveals quantitative patterns of fsh biodiversity in large rivers despite its downstream transportation	Pont et al.	2018
383	Detection of elusive fire salamander larvae (Salamandra salamandra) in streams via environmental DNA	Preißler et al.	2018
384	Effects of bioturbation on environmental DNA migration through soil media	Prosser and Hedgpeth	2018
385	Detection of the European pond turtle (Emys orbicularis) by environmental DNA: is eDNA adequate for reptiles?	Raemy et al.	2018
386	DNA-based monitoring of the alien invasive North American crayfish Procambarus clarkii in Andean lakes (Ecuador)	Riascos et al.	2018
387	An environmental DNA assay for the detection of the regionally endangered freshwater fish Alburnoides bipunctatus in Germany	Riaz et al.	2018
388	Environmental DNA detects a rare large river crayfish but with little relation to local abundance	Rice et al.	2018
389	Quantitative PCR assays for detection of five arctic fish species: Lota lota, Cottus cognatus, Salvelinus alpinus, Salvelinus malma, and Thymallus arcticus from environmental DNA	Rodgers et al.	2018
390	Monitoring post-release survival of the northern corroboree frog, Pseudophryne pengilleyi, using environmental DNA	Rojahn et al.	2018
391	eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity	Roussel et al.	2018
392	Development of environmental DNA (eDNA) methods for detecting high-risk freshwater fishes in live trade in Canada	Roy et al.	2018
393	Using environmental DNA to detect Hypophthalmichthys molitrix during the spawning period in the Yangtze River	Ruan et al.	2018
394	Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)	Rusch et al.	2018
395	Phylogenetic and environmental DNA insights into emerging aquatic parasites: implications for risk management	Sana et al.	2018
396	MitoFish and MiFish pipeline: a mitochondrial genome database of fish with an analysis pipeline for environmental DNA metabarcoding	Sato et al.	2018
397	Usefulness of environmental DNA for detecting Schistosoma mansoni occurrence sites in Madagascar.	Sato et al.	2018
398	Correlating sea lamprey density with environmental DNA detections in the lab	Schloesser et al.	2018
399	Inference of genetic marker concentrations from field surveys to detect environmental DNA using Bayesian updating	Schultz	2018
400	Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types	Sellers et al.	2018
401	Environmental DNA (eDNA): A Promising Biological Survey Tool for Aquatic Species Detection	Senapati et al.	2018
402	Tradeoffs of a portable, field-based environmental DNA platform for detecting invasive northern pike (Esox lucius) in Alaska	Sepulveda et al.	2018
403	Establishing detection thresholds for environmental DNA using receiver operator characteristic (ROC) curves	Serrao et al.	2018
404	Acidity promotes degradation of multi-species environmental DNA in lotic mesocosms	Seymour et al.	2018
405	Using Seawater to Document Coral-Zoothanthella Diversity: A New Approach to Coral Reef Monitoring Using Environmental DNA	Shinzato et al.	2018
406	Water flow and biofilm cover influence environmental DNA (eDNA) detection in recirculating streams	Shogren et al.	2018
407	Metabarcoding of shrimp stomach content: Harnessing a natural sampler for fish biodiversity monitoring	Siegenthaler et al.	2018
408	Fecal source tracking and eDNA profling in an urban creek following an extreme rain event	Staley et al.	2018
409	Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity	Stat et al.	2018
410	Metabarcoding of benthic ciliate communities shows high potential for environmental monitoring in salmon aquaculture	Stoeck et al.	2018
411	GoFish: A versatile nested PCR strategy for environmental DNA assays for marine vertebrates	Stoeckle et al.	2018
412	Utility of eDNA and occupancy models for monitoring an endangered fish across diverse riverine habitats	Strickland et al.	2018
413	Characterising planktonic dinoflagellate diversity in Singapore using DNA metabarcoding	Sze et al.	2018
414	ANDeTM: A fully integrated environmental DNA sampling system	Thomas et al.	2018
415	Concentrations of environmental DNA (eDNA) reflect spawning salmon abundance at fine spatial and temporal scales	Tillotsona et al.	2018
416	Environmental DNA sampling as a surveillance tool for cane toad Rhinella marina introductions on offshore islands	Tingley et al.	2018
417	Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species	Tsuji et al.	2018
418	Demonstration of the potential of environmental DNA as a tool for the detection of avian species	Ushio et al.	2018
419	Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing	Ushio et al.	2018
420	eDNA detection of corallivorous seastar (Acanthaster cf. solaris) outbreaks on the Great Barrier Reef using digital droplet PCR	Uthicke et al.	2018
421	Entomological signatures in honey: an environmental DNA metabarcoding approach can disclose information on plant- sucking insects in agricultural and forest landscapes	Utseri et al.	2018
422	Early detection of invasive exotic insect infestations using eDNA from crop surfaces	Valentin et al.	2018
423	Using eDNA, sediment subfossils, and zooplankton nets to detect invasive spiny water flea (Bythotrephes longimanus)	Walsh et al.	2018
424	A microcosm study of surface sediment environmental DNA: decay observation, abundance estimation, and fragment length comparison.	Wei et al.	2018
425	Effects of treated sample weight and DNA marker length on sediment eDNA based detection of a benthic invertebrate	Wei et al.	2018
426	Capture enrichment of aquatic environmental DNA: A first proof of concept	Wilcox et al.	2018
427	Comment: The Importance of Sound Methodology in Environmental DNA Sampling	Wilcox et al.	2018
428	Fine‐scale environmental DNA sampling reveals climate‐mediated interactions between native and invasive trout species	Wilcox et al.	2018
429	Detection and persistence of environmental DNA from an invasive, terrestrial mammal	Williams et al.	2018
430	Tracking the southern river terrapin (Batagur affinis) through environmental DNA: prospects and challenges	Wilson et al.	2018
431	Using environmental DNA and occupancy modelling to identify drivers of eastern hellbender (Cryptobranchus alleganiensis alleganiensis) extirpation	Wineland et al.	2018
432	Environmental DNA reveals nonmigratory individuals of Palaemon paucidens overwintering in Lake Biwa shallow waters	Wu et al.	2018
433	Early detection of a highly invasive bivalve based on environmental DNA (eDNA)	Xia et al.	2018
434	Conventional versus real‐time quantitative PCR for rare species detection	Xia et al.	2018
435	Monitoring seasonal distribution of an endangered anadromous sturgeon in a large river using environmental DNA	Xu et al.	2018
436	Species-specific detection of the endangered piscivorous cyprinid fish Opsariichthys uncirostris uncirostris, three-lips, using environmental DNA analysis	Yamanaka et al.	2018
437	Development of quantitative PCR assays for the detection and quantification of lake sturgeon (Acipenser fulvescens) environmental DNA	Yusishen et al.	2018
438	Advantages and Limitations of Environmental DNA/RNA Tools for Marine Biosecurity: Management and Surveillance of Non-indigenous Species	Zaiko et al.	2018

対象となった分類群(~2019年)

魚類に次いで両生類が検出対となることが多いようです。魚類は淡水魚、両生類はサンショウウオ類やイモリが多かった覚えがあります。

最終更新: 2020/10/5
情報元: Tsuji et al.2019

掲載ジャーナル(~2020年10月まで)

総合的な雑誌であるPLoSOneに次いで、分子生物学分野のMolecular Ecology Resources、 そして環境DNAを中心とした雑誌であるEnvironmental DNA JournalがTop3となっています。

最終更新: 2020/10/5
情報元: Tsuji et al.2019+α

環境DNAで検出できる淡水魚種

環境省生物多様性センターが発表した「環境DNA分析技術を用いた淡水魚類調査手法の手引き」に掲載されているMiFishプライマー領域のDNA配列の有無と種特異的プライマーに関する論文の有無についてまとめた表になります。 種特異的プライマーの整備状況は把握しているものについて追記しました。

気になる種について検索をかけて、種特異的プライマーの表記をクリックすると参考となる論文にリンクするようになっています。

• [Update] MiFish領域の有無の表記をクリックで、NCBI genbankに学名 + 12sで検索がかかるようにしました。

※ 複数種を対象としたユニバーサルプライマーについてはこちらを参照ください。

最終更新: 2023/2/12
参考: 生物多様性センター

No.	和名	学名	種特異的プライマー	MiFish領域の有無	MiFish法での識別 (◎ or △)
1	ミツバヤツメ	Entosphenus tridentatus		○	◎
2	カワヤツメ	Lethenteron japonicum		○	△
3	シベリアヤツメ	Lethenteron kessleri		○	△
4	スナヤツメ北方種	Lethenteron sp.N		○	◎
5	スナヤツメ南方種	Lethenteron sp.S	TaqMan	○	◎
6	チョウザメ	Acipenser medirostris		○	◎
7	アリゲーターガー	Atractosteus spatula		○	◎
8	スポッテッドガー	Lepisosteus oculatus		○	△
9	ロングノーズガー	Lepisosteus osseus		○	△
10	ショートノーズガー	Lepisosteus platostomus		○	△
11	ニューギニアウナギ	Anguilla bicolor pacifica		○	◎
12	ニホンウナギ	Anguilla japonica	TaqMan	○	◎
12	ニホンウナギ	Anguilla japonica	TaqMan	○	◎
13	オオウナギ	Anguilla marmorata	TaqMan	○	◎
14	コゲウツボ	Uropterygius concolor		○	◎
15	ナミダカワウツボ	Echidna rhodochilus		○	◎
16	ニシン	Clupea pallasii		○	◎
17	ドロクイ	Nematalosa japonica		○	◎
18	エツ	Coilia nasus		○	◎
19	コイ(飼育型)	Cyprinus carpio	TaqMan	○	◎
20	コイ(野生型)	Cyprinus carpio	TaqMan	○	◎
21	キンギョ	Carassius auratus	SYBR	○	△
22	オオキンブナ	Carassius buergeri buergeri		○	△
23	ニゴロブナ	Carassius buergeri grandoculis		○	△
24	ナガブナ	Carassius buergeri subsp.1		×	–
25	キンブナ	Carassius buergeri subsp.2		○	△
26	ゲンゴロウブナ	Carassius cuvieri		○	◎
27	ギンブナ	Carassius sp.		○	△
28	フナ属の 1 種(琉球列島)	Carassius sp.		○	△
29	ヤリタナゴ	Tanakia lanceolata		○	◎
30	ヤリタナゴ groupF(LA1)	Tanakia lanceolata		○	–
31	ヤリタナゴ groupE(LA2)	Tanakia lanceolata		○	–
32	ヤリタナゴ groupA(LA3)	Tanakia lanceolata		○	–
33	ヤリタナゴ groupG(LA4)	Tanakia lanceolata		○	–
34	ヤリタナゴ groupB	Tanakia lanceolata		○	–
35	ヤリタナゴ groupC	Tanakia lanceolata		○	–
36	ヤリタナゴ groupD-1	Tanakia lanceolata		○	–
37	ヤリタナゴ groupD-2	Tanakia lanceolata		○	–
38	ヤリタナゴ groupD-3	Tanakia lanceolata		×	–
39	アブラボテ	Tanakia limbata		○	◎
40	アブラボテ LI1	Tanakia limbata		○	–
41	アブラボテ LI2	Tanakia limbata		○	–
42	アブラボテ LI3	Tanakia limbata		○	–
43	ミヤコタナゴ	Tanakia tanago		○	◎
44	イチモンジタナゴ	Acheilognathus cyanostigma		○	◎
45	イチモンジタナゴ clade1	Acheilognathus cyanostigma		○	–
46	イチモンジタナゴ clade2	Acheilognathus cyanostigma		○	–
47	イチモンジタナゴ clade3	Acheilognathus cyanostigma		○	–
48	イタセンパラ	Acheilognathus longipinnis	SYBR	○	◎
49	イタセンパラ(琵琶湖-淀川型)	Acheilognathus longipinnis		○	–
50	イタセンパラ(濃尾型)	Acheilognathus longipinnis		○	–
51	イタセンパラ(富山型)	Acheilognathus longipinnis		×	–
52	オオタナゴ	Acheilognathus macropterus		○	◎
53	タナゴ	Acheilognathus melanogaster		○	◎
54	カネヒラ	Acheilognathus rhombeus		○	◎
55	アカヒレタビラ	Acheilognathus tabira erythropterus		○	◎
56	ミナミアカヒレタビラ	Acheilognathus tabira jordani		○	◎
57	セボシタビラ	Acheilognathus tabira nakamurae		○	◎
58	シロヒレタビラ	Acheilognathus tabira tabira		○	◎
59	キタノアカヒレタビラ	Acheilognathus tabira tohokuensis		○	◎
60	ゼニタナゴ	Acheilognathus typus	TaqMan	○	◎
61	カゼトゲタナゴ	Rhodeus atremius atremius	TaqMan	○	◎
62	スイゲンゼニタナゴ	Rhodeus atremius suigensis	TaqMan	○	◎
63	ニッポンバラタナゴ	Rhodeus ocellatus kurumeus		○	◎
64	ニッポンバラタナゴ(大阪型)	Rhodeus ocellatus kurumeus		○	–
65	ニッポンバラタナゴ(山陽型)	Rhodeus ocellatus kurumeus		○	–
66	ニッポンバラタナゴ(九州型)	Rhodeus ocellatus kurumeus		○	–
67	タイリクバラタナゴ	Rhodeus ocellatus ocellatus		○	◎
68	ハクレン	Hypophthalmichthys molitrix	TaqMan	○	△
69	コクレン	Aristichthys nobilis	TaqMan	○	△
70	ワタカ	Ischikauia steenackeri		○	◎
71	パールダニオ	Danio albolineatus		○	◎
72	ゼブラダニオ	Danio rerio	TaqMan(add)	○	◎
73	カワバタモロコ	Hemigrammocypris neglectus	TaqMan	○	◎
74	カワバタモロコ(本州-四国型)	Hemigrammocypris neglectus		○	–
75	カワバタモロコ(九州型)	Hemigrammocypris neglectus		○	–
76	ハス	Opsariichthys uncirostris uncirostris	TaqMan	○	◎
77	オイカワ	Zacco platypus	TaqMan(add)	○	◎
78	オイカワ group1	Zacco platypus		○	–
79	オイカワ group2	Zacco platypus		○	–
80	オイカワ group3	Zacco platypus		○	–
81	ヌマムツ	Nipponocypris sieboldii		○	◎
82	ヌマムツ group1	Nipponocypris sieboldii		○	–
83	ヌマムツ group2	Nipponocypris sieboldii		○	–
84	ヌマムツ group3	Nipponocypris sieboldii		○	–
85	カワムツ	Nipponocypris temminckii		○	◎
86	カワムツ group1	Nipponocypris temminckii		○	–
87	カワムツ group2	Nipponocypris temminckii		○	–
88	カワムツ group3	Nipponocypris temminckii		○	–
89	ヒナモロコ	Aphyocypris chinensis		○	◎
90	ソウギョ	Ctenopharhyngodon idellus	TaqMan	○	◎
91	アオウオ	Mylopharyngodon piceus	●(add)	○	◎
92	ヤマナカハヤ	Phoxinus lagowskii yamamotis		×	–
93	ヤチウグイ	Phoxinus perenurus sachalinensis		○	◎
94	アブラハヤ	Rhynchocypris lagowskii steindachneri		○	◎
95	タカハヤ	Rhynchocypris oxycephalus jouyi		○	◎
96	タカハヤ group1	Rhynchocypris oxycephalus jouyi		○	–
97	タカハヤ group2	Rhynchocypris oxycephalus jouyi		○	–
98	ジュウサンウグイ	Tribolodon brandtii brandtii		○	◎
99	ジュウサンウグイ TBB1	Tribolodon brandtii brandtii		○	–
100	ジュウサンウグイ TBB2	Tribolodon brandtii brandtii		×	–
101	マルタ	Tribolodon brandtii maruta		○	◎
102	ウグイ	Tribolodon hakonensis	SYBR(add)	○	◎
103	ウグイ group1	Tribolodon hakonensis		○	–
104	ウグイ group2	Tribolodon hakonensis		○	–
105	ウグイ group3	Tribolodon hakonensis		○	–
106	ウグイ group4	Tribolodon hakonensis		○	–
107	ウグイ group5	Tribolodon hakonensis		○	–
108	ウグイ group6	Tribolodon hakonensis		○	–
109	ウケクチウグイ	Tribolodon nakamurai		○	◎
110	エゾウグイ	Tribolodon sachalinensis		○	◎
111	モツゴ	Pseudorasbora parva	SYBR(add)	○	△
111	モツゴ	Pseudorasbora parva	SYBR	○	△
112	モツゴ group1	Pseudorasbora parva		○	–
113	モツゴ group2	Pseudorasbora parva		○	–
114	モツゴ group3	Pseudorasbora parva		○	–
115	モツゴ group4	Pseudorasbora parva		○	–
116	ウシモツゴ	Pseudorasbora pugnax		○	◎
117	シナイモツゴ	Pseudorasbora pumila		○	◎
118	アブラヒガイ	Sarcocheilichthys biwaensis		○	◎
119	ビワヒガイ	Sarcocheilichthys variegatus microoculus		○	△
120	カワヒガイ	Sarcocheilichthys variegatus variegatus		○	△
121	カワヒガイ(東海型)	Sarcocheilichthys variegatus variegatus		○	–
122	カワヒガイ(西日本型)	Sarcocheilichthys variegatus variegatus		○	–
123	ムギツク	Pungtungia herzi		○	◎
124	ホンモロコ	Gnathopogon caerulescens		○	△
125	タモロコ	Gnathopogon elongatus elongatus		○	△
126	タモロコ E1(西日本型)	Gnathopogon elongatus elongatus		○	–
127	タモロコ E2(東海型)	Gnathopogon elongatus elongatus		○	–
128	タモロコ E3(伊那型)	Gnathopogon elongatus elongatus		○	–
129	スワモロコ	Gnathopogon elongatus suwae		×	–
130	ヨドゼゼラ	Biwia yodoensis		○	◎
131	ゼゼラ	Biwia zezera		○	◎
132	ゼゼラ(岐阜型)	Biwia zezera		○	–
133	ゼゼラ(琵琶湖型)	Biwia zezera		○	–
134	ゼゼラ(山陽型)	Biwia zezera		×	–
135	ゼゼラ(九州型)	Biwia zezera		×	–
136	ナガレカマツカ	Pseudogobio agathonectris		○	◎
137	カマツカ	Pseudogobio esocinus		○	◎
138	スナゴカマツカ	Pseudogobio polystictus		○	◎
139	ツチフキ	Abbottina rivularis		○	◎
140	ツチフキ(大陸型)	Abbottina rivularis		○	–
141	ツチフキ(日本在来型)	Abbottina rivularis		○	–
142	ニゴイ	Hemibarbus barbus		○	△
143	コウライニゴイ	Hemibarbus labeo		○	△
144	ズナガニゴイ	Hemibarbus longirostris		○	◎
145	コウライモロコ	Squalidus biwae tsuchigae		○	△
146	スゴモロコ	Squalidus chankaensis biwae		○	△
147	イトモロコ	Squalidus gracilis gracilis		○	◎
148	イトモロコ group1	Squalidus gracilis gracilis		○	–
149	イトモロコ group2	Squalidus gracilis gracilis		○	–
150	イトモロコ group3	Squalidus gracilis gracilis		○	–
151	デメモロコ	Squalidus japonicus japonicus		○	△
152	デメモロコ(琵琶湖型)	Squalidus japonicus japonicus		○	–
153	デメモロコ(濃尾型)	Squalidus japonicus japonicus		○	–
154	アカヒレ	Tanichthys albonubes		○	◎
155	ドジョウ	Misgurnus anguillicaudatus	TaqMan	○	◎
156	ドジョウ(在来系統)	Misgurnus anguillicaudatus		○	◎
157	ドジョウ(大陸系統)	Misgurnus anguillicaudatus		○	◎
158	キタドジョウ	Misgurnus sp.Clade A		○	◎
159	シノビドジョウ	Misgurnus sp.IR		○	◎
160	ヒョウモンドジョウ	Misgurnus sp.OK		○	◎
161	カラドジョウ	Paramisgurnus dabryanus		○	◎
162	アリアケスジシマドジョウ	Cobitis kaibarai		○	◎
163	オオガタスジシマドジョウ	Cobitis magnostriata		○	△
164	ヤマトシマドジョウ	Cobitis matsubarae		○	◎
165	サンヨウコガタスジシマドジョウ	Cobitis minamorii minamorii		○	◎
166	ビワコガタスジシマドジョウ	Cobitis minamorii oumiensis		○	△
167	サンインコガタスジシマドジョウ	Cobitis minamorii saninensis		○	◎
168	トウカイコガタスジシマドジョウ	Cobitis minamorii tokaiensis		○	△
169	ヨドコガタスジシマドジョウ	Cobitis minamorii yodoensis		×	–
170	オオヨドシマドジョウ	Cobitis sakahoko		○	◎
171	ヒナイシドジョウ	Cobitis shikokuensis		○	◎
172	オオシマドジョウ	Cobitis sp.BIWAE type A		○	◎
173	ニシシマドジョウ	Cobitis sp.BIWAE type B		○	◎
174	ヒガシシマドジョウ	Cobitis sp.BIWAE type C		○	◎
175	トサシマドジョウ	Cobitis sp.BIWAE type D		○	◎
176	ヤマトシマドジョウ A 型	Cobitis sp.’yamato’ complex Type A		○	◎
177	オンガスジシマドジョウ	Cobitis striata fuchigamii		○	△
178	ハカタスジシマドジョウ	Cobitis striata hakataensis		○	△
179	チュウガタスジシマドジョウ	Cobitis striata striata		○	△
180	イシドジョウ	Cobitis takatsuensis		○	◎
181	タンゴスジシマドジョウ	Cobitis takenoi		○	△
182	アジメドジョウ	Niwaella delicata		○	◎
183	アジメドジョウ G(太平洋側型)	Niwaella delicata		○	–
184	アジメドジョウ S(日本海側型)	Niwaella delicata		○	–
185	フクドジョウ	Barbatula oreas		○	◎
186	ヒメドジョウ	Lefua costata		○	◎
187	エゾホトケドジョウ	Lefua costata nikkonis		○	◎
188	ホトケドジョウ	Lefua echigonia		○	◎
189	ホトケドジョウ(北陸型)	Lefua echigonia		×	–
190	ホトケドジョウ(近畿型)	Lefua echigonia		○	–
191	ホトケドジョウ(東海型)	Lefua echigonia		○	–
192	ホトケドジョウ(山形型)	Lefua echigonia		○	–
193	ホトケドジョウ(東北型)	Lefua echigonia		○	–
194	ホトケドジョウ(北関東型)	Lefua echigonia		○	–
195	ホトケドジョウ(南関東型)	Lefua echigonia		○	–
196	ホトケドジョウ(岩手型)	Lefua echigonia		○	–
197	ナガレホトケドジョウ	Lefua sp.1		○	◎
198	ナガレホトケドジョウ(紀伊-四国型)	Lefua sp.1		○	–
199	ナガレホトケドジョウ(山陽-山陰型)	Lefua sp.1		○	–
200	トウカイナガレホトケドジョウ	Lefua sp.2		○	◎
201	アユモドキ	Parabotia curtus	TaqMan	○	◎
202	アリアケギバチ	Tachysurus aurantiacus		○	◎
203	ネコギギ	Tachysurus ichikawai	TaqMan	○	◎
204	ギギ	Tachysurus nudiceps		○	◎
205	ギバチ	Tachysurus tokiensis		○	◎
206	コウライギギ	Pseudobagrus fulvidraco		○	◎
207	ナマズ	Silurus asotus		○	△
208	ビワコオオナマズ	Silurus biwaensis		○	◎
209	イワトコナマズ	Silurus lithophilus		○	△
210	タニガワナマズ	Silurus tomodai		○	△
211	アカザ	Liobagrus reinii	TaqMan	○	◎
212	アカザ Group1	Liobagrus reinii		○	–
213	アカザ Group2	Liobagrus reinii		○	–
214	チャネルキャットフィッシュ	Ictalurus punctatus		○	◎
215	ヒレナマズ	Clarias fuscus		○	◎
216	マダラロリカリア	Pterygoplichthys disjunctivus		○	△
217	シシャモ	Spirinchus lanceolatus	TaqMan	○	◎
218	キュウリウオ	Osmerus dentex		○	◎
219	ワカサギ	Hypomesus nipponensis	●(add)	○	◎
220	イシカリワカサギ	Hypomesus olidus		○	◎
221	アユ	Plecoglossus altivelis altivelis	TaqMan	○	◎
222	リュウキュウアユ	Plecoglossus altivelis ryukyuensis	TaqMan	○	◎
223	アリアケシラウオ	Salanx ariakensis		○	◎
224	アリアケヒメシラウオ	Neosalanx reganius		×	–
225	イトウ	Hucho perryi	TaqMan	○	◎
226	ブラウントラウト	Salmo trutta	●(add)	○	◎
227	カワマス	Salvelinus fontinalis	●(add)	○	◎
228	ゴギ	Salvelinus leucomaenis imbrius		○	△
229	ヤマトイワナ	Salvelinus leucomaenis japonicus		○	△
230	アメマス	Salvelinus leucomaenis leucomaenis	TaqMan	○	△
231	ニッコウイワナ	Salvelinus leucomaenis pluvius		○	△
232	オショロコマ	Salvelinus malma krascheninnikovi	TaqMan	○	△
233	ミヤベイワナ	Salvelinus malma miyabei		○	△
234	レイクトラウト	Salvelinus namaycush	●(add)	○	◎
235	カラフトマス	Oncorhynchus gorbuscha		○	◎
236	クニマス	Oncorhynchus kawamurae		×	–
237	サケ	Oncorhynchus keta	TaqMan(add)	○	△
238	サツキマス(アマゴ)	Oncorhynchus masou ishikawae		○	△
239	サクラマス(ヤマメ)	Oncorhynchus masou masou		○	△
240	ニジマス	Oncorhynchus mykiss	TaqMan	○	△
241	ベニザケ(ヒメマス)	Oncorhynchus nerka		○	◎
242	ビワマス	Oncorhynchus sp.		○	◎
243	タウナギ(本土産)	Monopterus albus		○	◎
244	タウナギ(沖縄産)	Monopterus sp.		○	◎
245	イトヨ	Gasterosteus aculeatus aculeatus	TaqMan	○	△
246	イトヨ(降海型)	Gasterosteus aculeatus aculeatus		×	–
247	イトヨ(福井型)	Gasterosteus aculeatus aculeatus		×	–
248	イトヨ(那須型)	Gasterosteus aculeatus aculeatus		○	–
249	太平洋系陸封型イトヨ	Gasterosteus aculeatus subsp.1		○	◎
250	ハリヨ	Gasterosteus aculeatus subsp.2		○	△
251	ハリヨ(濃尾型)	Gasterosteus aculeatus subsp.2		○	–
252	ハリヨ(近江型)	Gasterosteus aculeatus subsp.2		○	–
253	イトヨ湖沼型(福島県)	Gasterosteus aculeatus subsp.3		○	◎
254	ニホンイトヨ	Gasterosteus nipponicus		○	◎
255	ミナミトミヨ	Pungitius kaibarae		○	◎
256	トミヨ属淡水型	Pungitius sp.1		○	△
257	トミヨ属汽水型	Pungitius sp.2		○	△
258	トミヨ属雄物型	Pungitius sp.3		○	△
259	ムサシトミヨ	Pungitius sp.4		○	◎
260	エゾトミヨ	Pungitius tymensis		○	◎
261	アミメカワヨウジ	Hippichthys heptagonus		○	◎
262	ホシイッセンヨウジ	Microphis argulus		×	–
263	ヒメテングヨウジ	Microphis jagorii		×	–
264	タニヨウジ	Microphis retzii		×	–
265	カワボラ	Cestraeus plicatilis		○	◎
266	ナガレフウライボラ	Crenimugil heterocheilos		×	–
267	オニボラ	Ellochelon vaigiensis		○	◎
268	アンピンボラ	Chelon subviridis		○	◎
269	モンナシボラ	Moolgarda engeli		○	◎
270	カマヒレボラ	Moolgarda pedaraki		○	◎
271	ペヘレイ	Odontesthes bonariensis		○	◎
272	ネッタイイソイワシ	Atherinomorus duodecimalis		○	◎
273	ミナミギンイソイワシ	Hypoatherina temminckii		○	◎
274	グリーンソードテール	Xiphophorus hellerii		○	◎
275	カダヤシ	Gambusia affinis	TaqMan(add)	○	◎
276	グッピー	Poecilia reticulata	TaqMan(add)	○	◎
277	ミナミメダカ	Oryzias latipes	TaqMan	○	◎
277	ミナミメダカ	Oryzias latipes	TaqMan	○	◎
278	ミナミメダカ(ヒメダカ)	Oryzias latipes		○	◎
279	キタノメダカ	Oryzias sakaizumii	TaqMan	○	◎
280	コモチサヨリ	Zenarchopterus dunckeri		○	◎
281	クルメサヨリ	Hyporhamphus intermedius		○	◎
282	アゴヒゲオコゼ	Tetraroge barbata		○	◎
283	ヒゲソリオコゼ	Tetraroge nigra		×	–
284	アカメ	Lates japonicus		○	◎
285	インドタカサゴイシモチ	Pseudambassis ranga		○	◎
286	ナンヨウタカサゴイシモチ	Ambassis interrupta		○	◎
287	ハナダカタカサゴイシモチ	Ambassis macracanthus		×	–
288	オヤニラミ	Coreoperca kawamebari	TaqMan	○	◎
289	オヤニラミ group1	Coreoperca kawamebari		×	–
290	オヤニラミ group2	Coreoperca kawamebari		×	–
291	スズキ	Lateolabrax japonicus	TaqMan	○	△
292	シラヌイハタ	Epinephelus bontoides		○	◎
293	ブルーギル	Lepomis macrochirus	TaqMan	○	◎
294	コクチバス	Micropterus dolomieu	●(add)	○	◎
294	コクチバス	Micropterus dolomieu	TaqMan	○	◎
295	オオクチバス	Micropterus salmoides	TaqMan	○	◎
296	カガミテンジクダイ	Yarica hyalosoma		○	◎
297	ワキイシモチ	Fibramia lateralis		×	–
298	ヒルギヌメリテンジクダイ	Pseudamia amblyuroptera		○	◎
299	ウラウチフエダイ	Lutjanus goldiei		×	–
300	ダイダイコショウダイ	Plectorhinchus albovittatus		×	–
301	ナンヨウチヌ	Acanthopagrus pacificus		○	◎
302	アオギス	Sillago parvisquamis		○	◎
303	アトクギス	Sillaginops macrolepis		○	◎
304	テッポウウオ	Toxotes jaculatrix		○	◎
305	カワスズメ	Oreochromis mossambicus	●(add)	○	◎
306	ナイルティラピア	Oreochromis niloticus	●(add)	○	◎
307	ジルティラピア	Tilapia zillii		○	◎
308	ニセシマイサキ	Mesopristes argenteus		○	◎
309	ヨコシマイサキ	Mesopristes cancellatus		○	◎
310	シミズシマイサキ	Mesopristes iravi		○	◎
311	トゲナガユゴイ	Kuhlia munda		×	–
312	ヤマノカミ	Trachidermus fasciatus		○	◎
313	エゾハナカジカ	Cottus amblystomopsis		○	◎
314	カンキョウカジカ	Cottus hangiongensis		○	◎
315	カマキリ	Cottus kazika		○	◎
316	ハナカジカ	Cottus nozawae		○	◎
317	ハナカジカ(北海道型)	Cottus nozawae		○	–
318	ハナカジカ(北東北型)	Cottus nozawae		×	–
319	ハナカジカ(山形型)	Cottus nozawae		○	–
320	カジカ	Cottus pollux		○	△
321	カジカ小卵型	Cottus reinii		○	–
322	ウツセミカジカ(琵琶湖型)	Cottus reinii		○	–
323	ウツセミカジカ(回遊型)	Cottus reinii		○	–
324	カジカ中卵型	Cottus sp.ME	TaqMan(add)	○	△
325	ウラウチヘビギンポ	Enneapterygius cheni		×	–
326	ヒルギギンポ	Omox biporos		×	–
327	ゴマクモギンポ	Omobranchus elongatus		○	◎
328	カワギンポ	Omobranchus ferox		×	–
329	ナリタイトヒキヌメリ	Pseudocalliurichthys ikedai		×	–
330	ツバサハゼ	Rhyacichthys aspro		○	◎
331	イシドンコ	Odontobutis hikimius		○	◎
332	ドンコ	Odontobutis obscura		○	◎
333	ドンコ(西九州型)	Odontobutis obscura		○	–
334	ドンコ(西瀬戸内海型)	Odontobutis obscura		○	–
335	ドンコ(東瀬戸内海型)	Odontobutis obscura		○	–
336	ドンコ(山陰-琵琶湖-伊勢型)	Odontobutis obscura		○	–
337	タナゴモドキ	Hypseleotris cyprinoides		○	◎
338	オウギハゼ	Bunaka gyrinoides		○	△
339	テンジクカワアナゴ	Eleotris fusca		○	◎
340	カワアナゴ	Eleotris oxycephala		○	◎
341	エリトゲハゼ	Belobranchus belobranchus		○	◎
342	ヤエヤマノコギリハゼ	Butis amboinensis		○	◎
343	ジャノメハゼ	Bostrychus sinensis		○	◎
344	ホシマダラハゼ	Ophiocara porocephala		○	◎
345	タメトモハゼ	Giuris sp.1		○	◎
346	ゴシキタメトモハゼ	Giuris sp.2		×	–
347	ドウクツミミズハゼ	Luciogobius albus		×	–
348	ネムリミミズハゼ	Luciogobius dormitoris		×	–
349	ナガレミミズハゼ	Luciogobius fluvialis		×	–
350	ユウスイミミズハゼ	Luciogobius fonticola		○	◎
351	イドミミズハゼ	Luciogobius pallidus		○	◎
352	ミナミヒメミミズハゼ	Luciogobius ryukyuensis		○	△
353	ヒモハゼ	Eutaeniichthys gilli		○	◎
354	シロウオ	Leucopsarion petersii		○	◎
355	ワラスボ	Odontamblyopus lacepedii		○	◎
356	アサガラハゼ	Caragobius urolepis		○	◎
357	チワラスボ	Taenioides cirratus		○	△
358	ヒゲワラスボ	Trypauchenopsis intermedia		○	◎
359	トカゲハゼ	Scartelaos histophorus		○	◎
360	ムツゴロウ	Boleophthalmus pectinirostris		○	◎
361	タビラクチ	Apocryptodon punctatus		○	◎
362	トビハゼ	Periophthalmus modestus		○	◎
363	ヒメトサカハゼ	Cristatogobius aurimaculatus		×	–
364	トサカハゼ	Cristatogobius lophius		○	◎
365	クロトサカハゼ	Cristatogobius nonatoae		○	◎
366	シマサルハゼ	Oxyurichthys sp.2		×	–
367	ミスジハゼ	Callogobius sp.		×	–
368	ハゼクチ	Acanthogobius hasta		○	◎
369	ミナミアシシロハゼ	Acanthogobius insularis		○	◎
370	ヨロイボウズハゼ	Lentipes armatus		○	◎
371	カエルハゼ	Smilosicyopus leprurus		○	◎
372	ヒノコロモボウズハゼ	Sicyopus auxilimentus		×	–
373	アカボウズハゼ	Sicyopus zosterophorus		○	◎
374	ボウズハゼ	Sicyopterus japonicus		○	◎
375	ルリボウズハゼ	Sicyopterus lagocephalus		○	◎
376	ヒスイボウズハゼ	Stiphodon alcedo		○	◎
377	コンテリボウズハゼ	Stiphodon atropurpureus		×	–
378	ハヤセボウズハゼ	Stiphodon imperiorientis		○	◎
379	トラフボウズハゼ	Stiphodon multisquamus		×	–
380	ニライカナイボウズハゼ	Stiphodon niraikanaiensis		×	–
381	ナンヨウボウズハゼ	Stiphodon percnopterygionus		○	◎
382	カキイロヒメボウズハゼ	Stiphodon surrufus		×	–
383	ワカケサラサハゼ	Amblygobius linki		×	–
384	シラヌイハゼ	Silhouettea dotui		×	–
385	ニセシラヌイハゼ	Silhouettea sp.		×	–
386	ギンポハゼ	Parkraemeria saltator		○	◎
387	マングローブゴマハゼ	Pandaka lidwilli		○	◎
388	ゴマハゼ	Pandaka sp.		○	◎
389	カブキハゼ	Eugnathogobius mindora		×	–
390	ホホグロハゼ	Mugilogobius cavifrons		○	◎
391	フタホシハゼ	Mugilogobius fuscus		×	–
392	ムジナハゼ	Mugilogobius mertoni		×	–
393	コクチスナゴハゼ	Pseudogobius gastrospilos		○	◎
394	マサゴハゼ	Pseudogobius masago		○	◎
395	シマエソハゼ	Schismatogobius ampluvinculus		○	◎
396	エソハゼ	Schismatogobius roxasi		○	◎
397	ドウケハゼ	Stenogobius ophthalmoporus		×	–
398	タネカワハゼ	Stenogobius sp.		○	◎
399	クロミナミハゼ	Awaous melanocephalus		○	△
400	ショウキハゼ	Tridentiger barbatus		○	◎
401	シモフリシマハゼ	Tridentiger bifasciatus		○	◎
402	ヌマチチブ	Tridentiger brevispinis		○	△
403	ナガノゴリ	Tridentiger kuroiwae		○	△
404	シロチチブ	Tridentiger nudicervicus		○	◎
405	チチブ	Tridentiger obscurus		○	△
406	アカオビシマハゼ	Tridentiger trigonocephalus		○	◎
407	タスキヒナハゼ	Redigobius balteatus		○	◎
408	カワクモハゼ	Bathygobius sp.		×	–
409	ビワヨシノボリ	Rhinogobius biwaensis		○	△
410	クロヨシノボリ	Rhinogobius brunneus		○	△
411	カワヨシノボリ	Rhinogobius flumineus		○	△
412	カワヨシノボリ group1	Rhinogobius flumineus		○	–
413	カワヨシノボリ group2	Rhinogobius flumineus		○	–
414	カワヨシノボリ group3	Rhinogobius flumineus		○	–
415	オオヨシノボリ	Rhinogobius fluviatilis		○	△
416	クロダハゼ	Rhinogobius kurodai		○	△
417	ルリヨシノボリ	Rhinogobius mizunoi		○	△
418	シマヨシノボリ	Rhinogobius nagoyae		○	△
419	オガサワラヨシノボリ	Rhinogobius ogasawaraensis		○	△
420	ゴクラクハゼ	Rhinogobius similis		○	◎
421	アオバラヨシノボリ	Rhinogobius sp.BB		○	△
422	シマヒレヨシノボリ	Rhinogobius sp.BF		○	△
423	ヒラヨシノボリ	Rhinogobius sp.DL		○	△
424	カズサヨシノボリ	Rhinogobius sp.KZ		○	△
425	アヤヨシノボリ	Rhinogobius sp.MO		○	△
426	オウミヨシノボリ	Rhinogobius sp.OM		○	△
427	旧トウヨシノボリ類	Rhinogobius sp.OR		○	△
428	トウカイヨシノボリ	Rhinogobius sp.TO		○	◎
429	キバラヨシノボリ	Rhinogobius sp.YB		○	△
430	コンジキハゼ	Glossogobius aureus		○	◎
431	アゴヒゲハゼ	Glossogobius bicirrhosus		×	–
432	スダレウロハゼ	Glossogobius circumspectus		○	◎
433	フタゴハゼ	Glossogobius sp.		×	–
434	ニセツムギハゼ	Acentrogobius audax		×	–
435	ホクロハゼ	Acentrogobius caninus		○	◎
436	ホホグロスジハゼ	Acentrogobius suluensis		×	–
437	キララハゼ	Acentrogobius viridipunctatus		○	◎
438	ジュズカケハゼ	Gymnogobius castaneus		○	△
439	キセルハゼ	Gymnogobius cylindricus		○	◎
440	イサザ	Gymnogobius isaza		○	◎
441	エドハゼ	Gymnogobius macrognathos		○	◎
442	ヘビハゼ	Gymnogobius mororanus		○	◎
443	コシノハゼ	Gymnogobius nakamurae		×	–
444	シマウキゴリ	Gymnogobius opperiens		○	◎
445	スミウキゴリ	Gymnogobius petschiliensis		○	◎
446	クボハゼ	Gymnogobius scrobiculatus		○	◎
447	ムサシノジュズカケハゼ	Gymnogobius sp.1		○	◎
448	ホクリクジュズカケハゼ	Gymnogobius sp.2		○	△
449	ホクリクジュズカケハゼ group1	Gymnogobius sp.2		○	–
450	ホクリクジュズカケハゼ group2	Gymnogobius sp.2		○	–
451	シンジコハゼ	Gymnogobius taranetzi		○	△
452	チクゼンハゼ	Gymnogobius uchidai		○	◎
453	ウキゴリ	Gymnogobius urotaenia		○	◎
454	ウラウチイソハゼ	Eviota ocellifer		○	◎
455	ナミノコハゼ	Gobitrichinotus radiocularis		×	–
456	トンガスナハゼ	Kraemeria tongaensis		×	–
457	クジャクハゼ	Parioglossus caeruleolineatus		×	–
458	ヒメサツキハゼ	Parioglossus interruptus		×	–
459	マイコハゼ	Parioglossus lineatus		×	–
460	ボルネオハゼ	Parioglossus palustris		○	◎
461	コビトハゼ	Parioglossus rainfordi		×	–
462	コマチハゼ	Parioglossus taeniatus		×	–
463	チョウセンブナ	Macropodus ocellatus		○	◎
464	タイワンキンギョ	Macropodus opercularis		○	◎
465	カムルチー	Channa argus	TaqMan	○	◎
466	コウタイ	Channa asiatica		○	◎
467	タイワンドジョウ	Channa maculata		○	◎
468	クサフグ	Takifugu alboplumbeus		○	△
469	カタクチイワシ	Engraulis japonicus	TaqMan(add)	○	追加
470	マアジ	Trachurus japonicus	TaqMan(add)	○	追加
471	ボラ	Mugil cephalus	●(add)	○	追加
472	シラウオ	Salangichthys microdon	●(add)	○	追加
473	クロダイ	Acanthopagrus schlegelii	TaqMan(add)	○	追加
473	クロダイ	Acanthopagrus schlegelii	TaqMan(add)	○	追加
474	ホンベラ	Halichoeres tenuispinnis	TaqMan(add)	○	追加
475	イシダイ	Oplegnathus fasciatus	TaqMan(add)	○	追加

＜表中の凡例＞

1. MiFish 法での識別
   ◎：MiFish 法で種（もしくは亜種・系統）レベルの同定が可能な種
   △：MiFish 法で種（もしくは亜種・系統）レベルの同定が難しいと判断された種
2. MiFish 領域の有無
   ◎：登録配列あり、×：登録配列なし

• PDF tableからcsvへの変換コード BCJ-eDNAprimer-table

MiFishプライマーを用いた魚類相解析に係る誤同定チェックシート

MiFishプライマーでの魚類相調査の際、結果の精査時に使用することが望ましいサポートツールです。配列データの誤同定配列のチェックシートなどがまとめられています。

1. MiFishに係る誤同定チェックシート
   -> Excel LINK
2. MiFish法に係る誤同定チェックシートの使い方に関する解説書（2022年3月）
   -> PDF LINK
3. MiFish法における種の識別性を確認するための分子系統樹（2022年3月）
   -> PDF LINK
4. MiFish法における種・種内系統の識別性の判定結果一覧（2022年2月）
   -> EXCEL LINK
5. リファレンス（MiFish配列）登録状況及び種特異的プライマー 整備状況一覧（2022年2月）
   -> EXCEL LINK

最終更新: 2023/2/12
参考: 生物多様性センター

環境DNAで検出できる生物種(魚類以外)

魚類以外にも環境DNAによる検出事例が増えてきたのでまとめ始めました。

使用される際にはPrimer blastやTm calculatorなどで種特異性とアニーリング温度を確認し、文献中の条件に十分注意したうえでご使用ください。また、プローブの蛍光色素の選択にも注意して使用してください。

「種特異的プライマー」のリンクは論文へのリンクです。

最終更新: 2023/3/4

No.	分類	和名	学名	種特異的プライマー	F-seq	R-seq	Pr-seq	アニーリング温度(℃)
1	Insecta	ヒゲナガカワトビケラ	Stenopsyche marmorata	SYBR	非公開	非公開	–	非公開
2	Bivalvia	カワヒバリガイ	Limnoperna fortunei	TaqMan	5′-CATAGAACCCCAGCAGTTGAC A-3	5′-AACGAACCGCCGATTGAC-3′	5′- [FAM]-AGCTGCTTTATCTCTTC-[MBG]-3′	60
3	Bivalvia	ヤマトシジミ	Corbicula japonica	TaqMan	5′-GGTTTAGGGTAGGTGATGCTACTTTG-3′	5′-AAGGATTTGTTGATCCAGTTTCG-3′	5′-[FAM]-TTGTTTTTCATTTTCTTGGTCC-[NFQ]-[MGB]-3′	60
4	Decapoda	スジエビ	Palaemon paucidens	TaqMan	5′-AAGTCTAACCTGCCCACTGAGTTA-3′	5′-TTTAAGCCTTTTCACTTAAAGGTCA-3′	5′-[FAM]-ATGAGGGAAAAACTG-[NFQ]-[MGB]-3′	60
5	Reptiles	シマヘビ	Elaphe quadrivirgata	TaqMan	非公開	非公開	非公開	非公開
6	Reptiles	ヤマカガシ	Rhabdophis tigrinus	TaqMan	非公開	非公開	非公開	非公開
7	Reptiles	ニホンマムシ	Gloydius blomhoffii	TaqMan	非公開	非公開	非公開	非公開
8	Insecta	ヒメタイコウチ	Nepa hoffmanni	TaqMan	5′-ATAGGACGAGAAGACCCTGT-3’	5′-ATAGGATCAATAAAACACTCATCCG-3′	5′-[FAM]-TTGTTGGGGCGACAGGGAGA-[TAMRA]-3′	60
9	Echinodermata	アカナマコ(mt)	Apostichopus japonicus	SYBR	5′-GTCACAAACTGGGATGATATGGAG-3’	5′-TCCACTCAACCCTAAAGCCAA-3′	–	65
10	Echinodermata	アカナマコ(nu)	Apostichopus japonicus	SYBR	5′-AGGTGCTCCAGACATGGCTTTCCCA-3’	5′-AAGCAATATTGCCCACGCAGGAG-3′	–	60
11	Decapoda	ニホンザリガニ	Cambaroides japonicus	TaqMan	非公開	非公開	非公開	非公開
12	Decapoda	ウチダザリガニ	Pacifastacus leniusculus	TaqMan	非公開	非公開	非公開	非公開
13	Plant	オオカタナダモ	Egeria densa	TaqMan	5′-CATTTCTCCTTCATTGTATTCTTTCACA-3′	5′-ATTTCTATCTGTATCGTAGCCACCAA-3′	5′-[FAM]-CGGGTCCGAACAGAAATGCTTCTCTCT–[TAMRA]-3′	60
14	Bivalvia	マツカサガイ(クレード1の一部, 2)	Pronodularia japanensis	TaqMan	非公開	非公開	非公開	非公開
15	Plant	クロモ	Hydrilla verticillata	TaqMan	5′-TTTGCGCGAATATGTAGAACTTGT-3′	5′-GCCAAGGTTTTAGCACAGGAAA-3′	5′-[FAM]-ATTATTGTAGTGGATCTTCA-[NFQ]-[MGB]-3′	58
16	Decapoda	アメリカザリガニ	Procambarus clarkii	TaqMan	非公開	非公開	非公開	非公開
17	Amphibia	ウシガエル	Lithobates catesbeiana	TaqMan	非公開	非公開	非公開	非公開
18	Amphibia	オオサンショウウオ	Andrias japonicus	TaqMan	5′-CGGCGTTCTTCAACCATTG-3′	5′-AGCTCAAATTATTAAGGAGGTGGTTAA-3′	5′-[FAM]-ACACTCTTTTTAATTGCCCCAGT-[NFQ]-[MGB]-3′	60
19	Amphibia	チュウゴクオオサンショウウオ	Andrias davidianus	TaqMan	5′-AACACTCTTTTTAATTGCCCCAATAT-3′	5′-GTTCTATTTATCCTTGCATTATCCAGT-3′	5′-CACTAATCACCTCCTTAAT-3′ ※1	60
21	Decapoda	モクズガニ	Eriocheir japonica	TaqMan	5′-TGCTAAAAAAGGGAACCCACATAGA-3′	5′-GCGGGTATTATTATTCATAGAGTCGGT-3′	5′-[FAM]-AAGATACTCTTAGCGGTATAAA-[NFQ]-[MGB]-3′	60
22	Mammalia	カワネズミ	Chimarrogale platycephala	TaqMan	非公開	非公開	非公開	非公開
23	Scyphozoa	アカクラゲ	Chrysaora pacifica	TaqMan	5′-CCCAGATATGGCTTTTCCTAGA-3′	5′-TGAGTGAGCTTGTATAGCTGATA-3′	5′-[FAM]-TAGGATCCTCCCTAATTG-[NFQ]-[MGB]-3′	60
24	Insecta	タガメ	Kirkaldyia deyrolli	TaqMan	非公開	非公開	非公開	非公開
25	Amphibia	オオダイガハラサンショウウオ	Hynobius boulengeri	TaqMan	非公開	非公開	非公開	非公開
26	Mammalia	ヌートリア	Myocastor coypus	TaqMan	5′-CACTACAACAGCTTTTTCATCAATCAC-3′	5′-TTCCTCGTCCAATGTGGAAGT-3′	5′-[FAM]-TGATTAATCCGTTATATACACGCT-[NFQ]-[MGB]-3′	60

※1 : プローブの蛍光色素が論文で未記載

MiFishデータベースに登録されている魚種ごとの配列数

日本淡水魚を中心にMiFishデータベースに登録されている種に対する登録配列数をまとめました。MiFish領域の列が×でかつ、配列数が0のものは現状公開されているMiFishのデータベースには無いものになります。ただ更新が活発なのでNCBIには既にあるかもしれません。

ここで表記してるものに関してはNCBIにのみ登録配列があっても、今はあえて追加はしておりません。

最終更新: 2020/10/16
情報元: Miya et al. 2020

ID	和名	学名	MiFish 領域	MiFishDB 登録配列数
1	ミツバヤツメ	Entosphenus tridentatus	◎	2
2	スナヤツメ北方種	Lethenteron sp. N.	◎	8
3	スナヤツメ南方種	Lethenteron sp. S.	◎	6
4	シベリアヤツメ	Lethenteron kessleri	○	1
5	カワヤツメ	Lethenteron japonicum	○	1
6	チョウザメ	Acipenser medirostris	○	2
7	アリゲーターガー	Atractosteus spatula	◎	4
8	ロングノーズガー	Lepisosteus osseus	○	4
9	スポッテッドガー	Lepisosteus oculatus	○	5
10	ニューギニアウナギ	Anguilla bicolor pacifica	○	5
11	ニホンウナギ	Anguilla japonica	○	8
12	オオウナギ	Anguilla marmorata	◎	6
13	コゲウツボ	Uropterygius concolor	○	3
14	ナミダカワウツボ	Echidna rhodochilus	○	1
15	ニシン	Clupea pallasii	○	6
16	ドロクイ	Nematalosa japonica	○	2
17	エツ	Coilia nasus	○	7
18	コイ（飼育品種）	Cyprinus carpio	◎	19
19	コイ（ノゴイ）	Cyprinus carpio	○	19
20	ゲンゴロウブナ	Carassius cuvieri	◎	3
21	キンギョ	Carassius auratus	◎	14
22	ニゴロブナ	Carassius auratus grandoculis	○	2
23	ナガブナ	Carassius buergeri subsp. 1	×	–
24	キンブナ	Carassius auratus subsp KINBUNA	○	3
25	オオキンブナ	Carassius buergeri buergeri	○	–
26	ギンブナ	Carassius auratus langsdorfii	○	3
27	フナ属の一種（琉球列島）	Carassius sp.	×	–
28	ミヤコタナゴ	Tanakia tanago	◎	2
29	ヤリタナゴ	Tanakia lanceolata	◎	7
30	アブラボテ	Tanakia limbata	○	5
31	カネヒラ	Acheilognathus rhombeus	○	4
32	オオタナゴ	Acheilognathus macropterus	○	4
33	イチモンジタナゴ	Acheilognathus cyanostigma	◎	3
34	タナゴ	Acheilognathus melanogaster	○	1
35	イタセンパラ	Acheilognathus longipinnis	○	1
36	ミナミアカヒレタビラ	Acheilognathus tabira jordani	○	2
37	セボシタビラ	Acheilognathus tabira nakamurae	○	2
38	シロヒレタビラ	Acheilognathus tabira tabira	○	2
39	アカヒレタビラ	Acheilognathus tabira erythropterus	○	1
40	キタノアカヒレタビラ	Acheilognathus tabira tohokuensis	○	1
41	ゼニタナゴ	Acheilognathus typus	○	2
42	タイリクバラタナゴ	Rhodeus ocellatus ocellatus	◎	4
43	ニッポンバラタナゴ	Rhodeus ocellatus kurumeus	○	3
44	スイゲンゼニタナゴ	Rhodeus atremius suigensis	○	1
45	カゼトゲタナゴ	Rhodeus atremius atremius	○	2
46	ハクレン	Hypophthalmichthys molitrix	○	30
47	コクレン	Hypophthalmichthys nobilis	○	37
48	ワタカ	Ischikauia steenackeri	◎	2
49	パールダニオ	Danio albolineatus	○	1
50	ゼブラダニオ	Danio rerio	◎	9
51	カワバタモロコ	Hemigrammocypris neglectus	○	2
52	ハス	Opsariichthys uncirostris uncirostris	○	1
53	オイカワ	Zacco platypus	◎	13
54	カワムツ	Nipponocypris temminckii	○	5
55	ヌマムツ	Nipponocypris sieboldii	○	4
56	ヒナモロコ	Aphyocypris chinensis	◎	1
57	ソウギョ	Ctenopharyngodon idella	○	5
58	アオウオ	Mylopharyngodon piceus	○	7
59	ヤチウグイ	Rhynchocypris percnurus sachalinensis	◎	5
60	アブラハヤ	Rhynchocypris lagowskii steindachneri	◎	6
61	ヤマナカハヤ	Phoxinus lagowskii yamamotis	×	–
62	タカハヤ	Rhynchocypris oxycephalus jouyi	◎	8
63	ジュウサンウグイ	Tribolodon brandtii brandtii	○	2
64	マルタ	Tribolodon brandtii maruta	○	1
65	ウケクチウグイ	Tribolodon nakamurai	○	1
66	エゾウグイ	Tribolodon sachalinensis	○	5
67	ウグイ	Tribolodon hakonensis	◎	3
68	モツゴ	Pseudorasbora parva	○	4
69	シナイモツゴ	Pseudorasbora pumila	◎	3
70	ウシモツゴ	Pseudorasbora pugnax	○	1
71	アブラヒガイ	Sarcocheilichthys biwaensis	○	1
72	カワヒガイ	Sarcocheilichthys variegatus variegatus	○	2
73	ビワヒガイ	Sarcocheilichthys variegatus microoculus	○	2
74	ムギツク	Pungtungia herzi	○	3
75	タモロコ	Gnathopogon elongatus elongatus	◎	6
76	スワモロコ	Gnathopogon elongatus suwae	×	–
77	ホンモロコ	Gnathopogon caerulescens	○	1
78	ゼゼラ	Biwia zezera	○	3
79	ヨドゼゼラ	Biwia yodoensis	○	1
80	カマツカ	Pseudogobio esocinus	◎	10
81	ナガレカマツカ	Pseudogobio agathonectris	◎	1
82	スナゴカマツカ	Pseudogobio polysticta	○	1
83	ツチフキ	Abbottina rivularis	◎	13
84	ズナガニゴイ	Hemibarbus longirostris	○	2
85	コウライニゴイ	Hemibarbus labeo	◎	6
86	ニゴイ	Hemibarbus barbus	◎	2
87	イトモロコ	Squalidus gracilis gracilis	◎	3
88	デメモロコ	Squalidus japonicus japonicus	○	3
89	スゴモロコ	Squalidus chankaensis biwae	◎	3
90	コウライモロコ	Squalidus chankaensis tsuchigae	○	5
91	アカヒレ	Tanichthys albonubes	○	1
92	ドジョウ	Misgurnus anguillicaudatus	◎	26
93	キタドジョウ	Misgurnus sp CladeA	◎	8
94	ヒョウモンドジョウ	Misgurnus sp OK	×	–
95	シノビドジョウ	Misgurnus sp IR	◎	1
96	ドジョウ（大陸産）	Misgurnus anguillicaudatus Clade B-1	○	–
97	カラドジョウ	Paramisgurnus dabryanus	◎	10
98	オオシマドジョウ	Cobitis sp BIWAE typeA	○	5
99	ニシシマドジョウ	Cobitis sp BIWAE typeB	◎	12
100	ヒガシシマドジョウ	Cobitis sp BIWAE typeC	○	5
101	トサシマドジョウ	Cobitis sp BIWAE typeD	×	2
102	サンヨウコガタスジシマドジョウ	Cobitis minamorii minamorii	○	2
103	トウカイコガタスジシマドジョウ	Cobitis minamorii tokaiensis	○	3
104	ビワコガタスジシマドジョウ	Cobitis minamorii oumiensis	○	1
105	ヨドコガタスジシマドジョウ	Cobitis minamorii yodoensis	×	–
106	サンインコガタスジシマドジョウ	Cobitis minamorii saninensis	○	1
107	チュウガタスジシマドジョウ	Cobitis striata striata	○	2
108	オンガスジシマドジョウ	Cobitis striata fuchigamii	×	1
109	ハカタスジシマドジョウ	Cobitis striata hakataensis	×	1
110	アリアケスジシマドジョウ	Cobitis kaibarai	◎	2
111	オオガタスジシマドジョウ	Cobitis magnostriata	○	1
112	タンゴスジシマドジョウ	Cobitis takenoi	○	1
113	ヤマトシマドジョウ	Cobitis matsubarae	◎	2
114	ヤマトシマドジョウA型	Cobitis sp ‘yamato’ complex typeA	×	1
115	オオヨドシマドジョウ	Cobitis sakahoko	○	1
116	イシドジョウ	Cobitis takatsuensis	◎	3
117	ヒナイシドジョウ	Cobitis shikokuensis	◎	1
118	アジメドジョウ	Niwaella delicata	◎	3
119	フクドジョウ	Barbatula barbatula	◎	2
120	エゾホトケドジョウ	Lefua nikkonis	◎	2
121	ヒメドジョウ	Lefua costata	○	3
122	ホトケドジョウ	Lefua echigonia	○	7
123	ナガレホトケドジョウ	Lefua torrentis	○	2
124	トウカイナガレホトケドジョウ	Lefua tokaiensis	○	1
125	アユモドキ	Parabotia curta	◎	2
126	ギギ	Tachysurus nudiceps	◎	1
127	ネコギギ	Tachysurus ichikawai	◎	1
128	ギバチ	Tachysurus tokiensis	○	3
129	アリアケギバチ	Tachysurus aurantiacus	×	1
130	コウライギギ	Tachysurus fulvidraco	○	3
131	イワトコナマズ	Silurus lithophilus	○	1
132	タニガワナマズ	Silurus tomodai	○	1
133	ビワコオオナマズ	Silurus biwaensis	○	1
134	ナマズ	Silurus asotus	◎	13
135	アカザ	Liobagrus reinii	◎	1
136	チャネルキャットフィッシュ	Ictalurus punctatus	○	3
137	ヒレナマズ	Clarias fuscus	○	3
138	マダラロリカリア	Pterygoplichthys disjunctivus	○	2
139	シシャモ	Spirinchus lanceolatus	○	2
140	キュウリウオ	Osmerus dentex	○	1
141	ワカサギ	Hypomesus nipponensis	○	1
142	イシカリワカサギ	Hypomesus olidus	○	1
143	アユ	Plecoglossus altivelis altivelis	◎	3
144	リュウキュウアユ	Plecoglossus altivelis ryukyuensis	×	–
145	アリアケシラウオ	Salanx ariakensis	○	2
146	アリアケヒメシラウオ	Neosalanx reganius	×	–
147	イトウ	Hucho perryi	○	1
148	ブラウントラウト	Salmo trutta	○	5
149	カワマス	Salvelinus fontinalis	○	4
150	レイクトラウト	Salvelinus namaycush	○	2
151	アメマス	Salvelinus leucomaenis leucomaenis	◎	1
152	ヤマトイワナ	Salvelinus leucomaenis japonicus	○	2
153	ニッコウイワナ	Salvelinus leucomaenis pluvius	◎	2
154	ゴギ	Salvelinus leucomaenis imbrius	○	1
155	オショロコマ	Salvelinus malma krascheninnikovi	○	1
156	ミヤベイワナ	Salvelinus malma miyabei	×	1
157	ニジマス	Oncorhynchus mykiss	○	10
158	サケ	Oncorhynchus keta	○	4
159	ヒメマス	Oncorhynchus nerka	○	6
160	クニマス	Oncorhynchus kawamurae	×	–
161	カラフトマス	Oncorhynchus gorbuscha	○	4
162	サクラマス（ヤマメ）	Oncorhynchus masou masou	◎	2
163	サツキマス（アマゴ）	Oncorhynchus masou ishikawae	○	2
164	ビワマス	Oncorhynchus masou rhodurus	◎	1
165	タウナギ（本土産）	Monopterus albus	○	5
166	タウナギ（沖縄産）	Monopterus sp.	×	–
167	イトヨ	Gasterosteus aculeatus aculeatus	○	1
168	ハリヨ	Gasterosteus aculeatus microcephalus	○	1
169	ニホンイトヨ	Gasterosteus nipponicus	○	1
170	エゾトミヨ	Pungitius tymensis	○	3
171	ミナミトミヨ	Pungitius kaibarae	○	4
172	トミヨ属雄物型	Pungitius sp Omono	○	1
173	トミヨ属淡水型	Pungitius sp Freshwater	○	1
174	トミヨ属汽水型	Pungitius sp Brackish	○	1
175	ムサシトミヨ	Pungitius sp MUSASHI TOMIYO	○	1
176	アミメカワヨウジ	Hippichthys heptagonus	◎	2
177	ホシイッセンヨウジ	Microphis argulus	×	–
178	ヒメテングヨウジ	Microphis jagorii	○	–
179	タニヨウジ	Microphis retzii	×	–
180	カワボラ	Cestraeus plicatilis	○	1
181	ナガレフウライボラ	Crenimugil heterocheilos	×	–
182	オニボラ	Ellochelon vaigiensis	◎	3
183	アンピンボラ	Chelon subviridis	◎	1
184	カマヒレボラ	Moolgarda pedaraki	◎	3
185	モンナシボラ	Moolgarda engeli	○	1
186	ペヘレイ	Odontesthes bonariensis	○	2
187	ネッタイイソイワシ	Atherinomorus duodecimalis	◎	2
188	ミナミギンイソイワシ	Hypoatherina temminckii	○	2
189	グリーンソードテール	Xiphophorus hellerii	◎	3
190	カダヤシ	Gambusia affinis	○	2
191	グッピー	Poecilia reticulata	○	4
192	ミナミメダカ	Oryzias latipes	◎	18
193	キタノメダカ	Oryzias sakaizumii	○	9
194	コモチサヨリ	Zenarchopterus dunckeri	◎	3
195	クルメサヨリ	Hyporhamphus intermedius	○	2
196	アゴヒゲオコゼ	Tetraroge barbata	◎	1
197	ヒゲソリオコゼ	Tetraroge nigra	×	–
198	アカメ	Lates japonicus	◎	3
199	インドタカサゴイシモチ	Pseudambassis ranga	◎	–
200	ナンヨウタカサゴイシモチ	Ambassis interrupta	×	1
201	ハナダカタカサゴイシモチ	Ambassis macracanthus	×	–
202	オヤニラミ	Coreoperca kawamebari	○	2
203	スズキ	Lateolabrax japonicus	◎	5
204	シラヌイハタ	Epinephelus bontoides	◎	2
205	ブルーギル	Lepomis macrochirus	○	5
206	オオクチバス	Micropterus salmoides	◎	3
207	コクチバス	Micropterus dolomieu	◎	3
208	カガミテンジクダイ	Yarica hyalosoma	○	–
209	ワキイシモチ	Fibramia lateralis	○	–
210	ヒルギヌメリテンジクダイ	Pseudamia amblyuroptera	◎	1
211	ウラウチフエダイ	Lutjanus goldiei	×	–
212	ダイダイコショウダイ	Plectorhinchus albovittatus	×	–
213	ナンヨウチヌ	Acanthopagrus pacificus	◎	1
214	アオギス	Sillago parvisquamis	○	2
215	アトクギス	Sillaginops macrolepis	×	1
216	テッポウウオ	Toxotes chatareus	×	2
217	カワスズメ	Oreochromis mossambicus	○	1
218	ナイルティラピア	Oreochromis niloticus	○	11
219	ジルティラピア	Coptodon zillii	○	2
220	ヨコシマイサキ	Mesopristes cancellatus	○	2
221	ニセシマイサキ	Mesopristes argenteus	×	1
222	シミズシマイサキ	Mesopristes iravi	×	1
223	トゲナガユゴイ	Kuhlia munda	×	–
224	ヤマノカミ	Trachidermus fasciatus	○	2
225	カマキリ	Cottus kazika	○	3
226	カジカ	Cottus pollux	◎	6
227	カジカ中卵型	Cottus sp ME	○	1
228	カジカ小卵型	Cottus reinii	◎	4
229	カンキョウカジカ	Cottus hangiongensis	○	3
230	ハナカジカ	Cottus nozawae	◎	3
231	エゾハナカジカ	Cottus amblystomopsis	○	4
232	ウラウチヘビギンポ	Enneapterygius cheni	×	–
233	ヒルギギンポ	Omox biporos	×	–
234	ゴマクモギンポ	Omobranchus elongatus	◎	1
235	カワギンポ	Omobranchus ferox	×	–
236	ナリタイトヒキヌメリ	Pseudocalliurichthys ikedai	×	–
237	ツバサハゼ	Rhyacichthys aspro	○	2
238	ドンコ	Odontobutis obscura	◎	10
239	イシドンコ	Odontobutis hikimius	○	1
240	タナゴモドキ	Hypseleotris cyprinoides	◎	1
241	オウギハゼ	Bunaka gyrinoides	×	1
242	カワアナゴ	Eleotris oxycephala	○	5
243	テンジクカワアナゴ	Eleotris fusca	◎	4
244	エリトゲハゼ	Belobranchus belobranchus	×	–
245	ヤエヤマノコギリハゼ	Butis amboinensis	○	1
246	ジャノメハゼ	Bostrychus sinensis	○	6
247	ホシマダラハゼ	Ophiocara porocephala	◎	2
248	タメトモハゼ	Ophieleotris sp TAMETOMO HAZE	◎	1
249	ゴシキタメトモハゼ	Giuris sp. 2	×	–
250	ドウクツミミズハゼ	Luciogobius albus	×	–
251	ネムリミミズハゼ	Luciogobius dormitoris	×	–
252	イドミミズハゼ	Luciogobius pallidus	○	2
253	ナガレミミズハゼ	Luciogobius fluvialis	×	–
254	ユウスイミミズハゼ	Luciogobius fonticola	×	1
255	ミナミヒメミミズハゼ	Luciogobius ryukyuensis	○	2
256	ヒモハゼ	Eutaeniichthys gilli	◎	1
257	シロウオ	Leucopsarion petersii	○	4
258	ワラスボ	Odontamblyopus lacepedii	○	1
259	アサガラハゼ	Caragobius urolepis	○	1
260	チワラスボ	Taenioides cirratus	○	2
261	ヒゲワラスボ	Trypauchenopsis intermedia	○	2
262	トカゲハゼ	Scartelaos histophorus	○	1
263	ムツゴロウ	Boleophthalmus pectinirostris	○	3
264	タビラクチ	Apocryptodon punctatus	○	1
265	トビハゼ	Periophthalmus modestus	◎	4
266	トサカハゼ	Cristatogobius lophius	◎	2
267	ヒメトサカハゼ	Cristatogobius aurimaculatus	×	–
268	クロトサカハゼ	Cristatogobius nonatoae	○	–
269	シマサルハゼ	Oxyurichthys sp. 2	×	–
270	ミスジハゼ	Callogobius sp MISUJI HAZE	×	1
271	ハゼクチ	Acanthogobius hasta	○	6
272	ミナミアシシロハゼ	Acanthogobius insularis	×	1
273	ヨロイボウズハゼ	Lentipes armatus	◎	1
274	カエルハゼ	Smilosicyopus leprurus	×	–
275	アカボウズハゼ	Sicyopus zosterophorum	×	1
276	ヒノコロモボウズハゼ	Sicyopus auxilimentus	×	–
277	ルリボウズハゼ	Sicyopterus lagocephalus	◎	5
278	ボウズハゼ	Sicyopterus japonicus	◎	7
279	カキイロヒメボウズハゼ	Stiphodon surrufus	×	–
280	ナンヨウボウズハゼ	Stiphodon percnopterygionus	◎	2
281	ハヤセボウズハゼ	Stiphodon imperiorientis	×	–
282	コンテリボウズハゼ	Stiphodon atropurpureus	×	–
283	ヒスイボウズハゼ	Stiphodon alcedo	○	1
284	ニライカナイボウズハゼ	Stiphodon niraikanaiensis	×	–
285	トラフボウズハゼ	Stiphodon multisquamus	×	–
286	ワカケサラサハゼ	Amblygobius linki	×	–
287	シラヌイハゼ	Silhouettea dotui	×	–
288	ニセシラヌイハゼ	Silhouettea sp.	×	–
289	ギンポハゼ	Parkraemeria saltator	◎	1
290	マングローブゴマハゼ	Pandaka lidwilli	◎	1
291	ゴマハゼ	Pandaka sp.	×	–
292	カブキハゼ	Eugnathogobius mindora	×	–
293	フタホシハゼ	Mugilogobius fuscus	×	–
294	ホホグロハゼ	Mugilogobius cavifrons	○	1
295	ムジナハゼ	Mugilogobius mertoni	×	1
296	マサゴハゼ	Pseudogobius masago	◎	2
297	コクチスナゴハゼ	Pseudogobius sp KOKUCHI SUNAGO HAZE	×	1
298	エソハゼ	Schismatogobius roxasi	◎	1
299	シマエソハゼ	Schismatogobius ampluvinculus	×	24
300	タネカワハゼ	Stenogobius sp TANEKAWA HAZE	◎	1
301	ドウケハゼ	Stenogobius ophthalmoporus	×	–
302	クロミナミハゼ	Awaous melanocephalus	○	1
303	シロチチブ	Tridentiger nudicervicus	×	–
304	ショウキハゼ	Tridentiger barbatus	○	3
305	アカオビシマハゼ	Tridentiger trigonocephalus	◎	5
306	シモフリシマハゼ	Tridentiger bifasciatus	○	4
307	ヌマチチブ	Tridentiger brevispinis	○	2
308	チチブ	Tridentiger obscurus	◎	5
309	ナガノゴリ	Tridentiger kuroiwae	◎	2
310	タスキヒナハゼ	Redigobius balteatus	○	1
311	カワクモハゼ	Bathygobius sp.	×	–
312	ヒラヨシノボリ	Rhinogobius sp.DL	×	–
313	カワヨシノボリ	Rhinogobius flumineus	○	3
314	シマヨシノボリ	Rhinogobius nagoyae	◎	2
315	ルリヨシノボリ	Rhinogobius mizunoi	◎	1
316	アヤヨシノボリ	Rhinogobius sp MO	○	1
317	オオヨシノボリ	Rhinogobius fluviatilis	◎	2
318	クロヨシノボリ	Rhinogobius brunneus	○	9
319	オガサワラヨシノボリ	Rhinogobius ogasawaraensis	○	1
320	ゴクラクハゼ	Rhinogobius giurinus	◎	8
321	アオバラヨシノボリ	Rhinogobius sp BB	×	–
322	トウカイヨシノボリ	Rhinogobius sp TO	○	1
323	クロダハゼ	Rhinogobius kurodai	×	–
324	シマヒレヨシノボリ	Rhinogobius nagoyae	○	2
325	キバラヨシノボリ	Rhinogobius sp YB	○	1
326	ビワヨシノボリ	Rhinogobius biwaensis	◎	1
327	カズサヨシノボリ	Rhinogobius sp KZ	○	2
328	オウミヨシノボリ	Rhinogobius sp OM	○	1
329	旧トウヨシノボリ類	Rhinogobius sp OR	◎	4
330	アゴヒゲハゼ	Glossogobius bicirrhosus	×	–
331	スダレウロハゼ	Glossogobius circumspectus	×	2
332	コンジキハゼ	Glossogobius aureus	○	2
333	フタゴハゼ	Glossogobius sp	×	1
334	ホクロハゼ	Acentrogobius caninus	○	4
335	キララハゼ	Acentrogobius viridipunctatus	×	–
336	ニセツムギハゼ	Acentrogobius audax	×	–
337	ホホグロスジハゼ	Acentrogobius suluensis	×	–
338	イサザ	Gymnogobius isaza	○	1
339	スミウキゴリ	Gymnogobius petschiliensis	◎	4
340	ウキゴリ	Gymnogobius urotaenia	◎	6
341	シマウキゴリ	Gymnogobius opperiens	◎	3
342	ヘビハゼ	Gymnogobius mororanus	○	2
343	シンジコハゼ	Gymnogobius taranetzi	○	–
344	ジュズカケハゼ	Gymnogobius castaneus	○	6
345	コシノハゼ	Gymnogobius nakamurae	×	–
346	ムサシノジュズカケハゼ	Gymnogobius sp1 MUSASHINO JUZUKAKE HAZE	○	1
347	ホクリクジュズカケハゼ	Gymnogobius sp HOKURIKU JUZUKAKE HAZE	○	1
348	チクゼンハゼ	Gymnogobius uchidai	○	2
349	クボハゼ	Gymnogobius scrobiculatus	○	1
350	キセルハゼ	Gymnogobius cylindricus	×	1
351	エドハゼ	Gymnogobius macrognathos	○	1
352	ウラウチイソハゼ	Eviota ocellifer	×	1
353	ナミノコハゼ	Gobitrichinotus radiocularis	×	–
354	トンガスナハゼ	Kraemeria tongaensis	×	–
355	マイコハゼ	Parioglossus lineatus	×	–
356	コマチハゼ	Parioglossus taeniatus	×	–
357	ボルネオハゼ	Parioglossus palustris	×	1
358	コビトハゼ	Parioglossus rainfordi	×	–
359	ヒメサツキハゼ	Parioglossus interruptus	×	–
360	クジャクハゼ	Parioglossus caeruleolineatus	×	–
361	タイワンキンギョ	Macropodus opercularis	○	2
362	チョウセンブナ	Macropodus ocellatus	○	2
363	コウタイ	Channa asiatica	○	3
364	タイワンドジョウ	Channa maculata	○	4
365	カムルチー	Channa argus	◎	16
366	クサフグ	Takifugu alboplumbeus	◎	1
377	カタクチイワシ	Engraulis japonicus	Y	5
378	マアジ	Trachurus japonicus	Y	6
379	ボラ	Mugil cephalus	Y	16

※注意点
作業が半自動で、なおかつデータごとに学名表記の揺らぎがあるので、配列数は必ずしも正しいものではありません。また、NCBIの配列データ更新が活発なので、すぐに配列数は変わると思います。